Skip to Main content Skip to Navigation
Journal articles

Characterization of the Secretomes of Two Vibrios Pathogenic to Mollusks

Abstract : Vibrio tapetis causes the brown ring disease in the Japanese clam Ruditapes philippinarum while Vibrio aestuarianus is associated with massive oyster mortalities. As extracellular proteins are often associated with the virulence of pathogenic bacteria, we undertook a proteomic approach to characterize the secretomes of both vibrios. The extracellular proteins (ECPs) of both species were fractionated by SEC-FPLC and in vitro assays were performed to measure the effects of each fraction on hemocyte cellular parameters (phagocytosis and adhesion). Fractions showing a significant effect were subjected to SDS-PAGE, and proteins were identified by nano LC-MS/MS. 45 proteins were identified for V. aestuarianus and 87 for V. tapetis. Most of them belonged to outer membrane or were periplasmic, including porins or adhesins that were already described as virulence factors in other bacterial species. Others were transporter components, flagella proteins, or proteins of unknown function (14 and 15 respectively). Interestingly, for V. aestuarianus, we noted the secretion of 3 extracellular enzymes including the Vam metalloprotease and two other enzymes (one putative lipase and one protease). For V. tapetis, we identified five extracellular enymes, i.e. two different endochitinases, one protease, one lipase and an adhesin. A comparison of both secretomes also showed that only the putative extracellular lipase was common to both secretomes, underscoring the difference in pathogenicity mechanisms between these two species. Overall, these results characterize for the first time the secretomes of these two marine pathogenic vibrios and constitute a useful working basis to further analyze the contribution of specific proteins in the virulence mechanisms of these species. Citation: Madec S, Pichereau V, Jacq A, Paillard M, Boisset C, et al. (2014) Characterization of the Secretomes of Two Vibrios Pathogenic to Mollusks. PLoS ONE 9(11): e113097.
Document type :
Journal articles
Complete list of metadatas

Cited literature [40 references]  Display  Hide  Download

https://hal.univ-brest.fr/hal-01096899
Contributor : Adminhal Univbrestbu <>
Submitted on : Thursday, December 18, 2014 - 2:08:38 PM
Last modification on : Wednesday, September 16, 2020 - 4:58:32 PM
Long-term archiving on: : Monday, March 23, 2015 - 4:43:51 PM

File

Plos-Madec-2014pdf.pdf
Publisher files allowed on an open archive

Identifiers

Collections

Citation

Stéphanie Madec, Vianney Pichereau, Annick Jacq, Mathieu Paillard, Claire Boisset, et al.. Characterization of the Secretomes of Two Vibrios Pathogenic to Mollusks. PLoS ONE, Public Library of Science, 2014, 9 (11), pp.0113097. ⟨10.1371/journal.pone.0113097⟩. ⟨hal-01096899⟩

Share

Metrics

Record views

1047

Files downloads

1149