Transcriptional analysis of the cyclopropane fatty acid synthase gene of Lactococcus lactis MG1363 at low pH.

Aurélie Budin-Verneuil 1 Emmanuelle Maguin 2 Yanick Auffray 1 S Dusko Ehrlich 3 Vianney Pichereau 4, *
* Auteur correspondant
1 U2RM - Unité de Recherche Risques Microbiens
2 MICALIS - MICrobiologie de l'ALImentation au Service de la Santé humaine
3 1Génétique Microbienne, INRA, Domaine de Vilvert, 78352 Jouy en Josas Cedex
4 LEMAR - Laboratoire des Sciences de l'Environnement Marin (LEMAR)
Abstract : Cyclopropane fatty acid synthase (cfa) catalyses the transfer of a methyl group from S-adenosylmethionine (SAM) to unsaturated fatty acids. Northern blot experiments demonstrated that the Lactococcus lactis MG1363 cfa gene is mainly expressed as a bicistronic transcript together with metK, the gene encoding SAM synthetase, and is highly induced by acidity. The cfa promoter was characterized by 5'-RACE PCR, and fused to beta-galactosidase by cloning into the pAK80 plasmid. This transcriptional fusion was highly induced by acidity (23-fold at pH 5) as well as during entry into the stationary phase (8-fold) in L. lactis. Interestingly, the cfa promoter expression is repressed in a L. lactis relA* mutant which accumulates (p)ppGpp, whereas its induction by acidity appeared independent of (p)ppGpp in L. lactis and in Escherichia coli.
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Article dans une revue
FEMS Microbiology Letters, Wiley-Blackwell, 2005, 250 (2), pp.189-94. 〈10.1016/j.femsle.2005.07.007〉
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http://hal.univ-brest.fr/hal-01067008
Contributeur : Vianney Pichereau <>
Soumis le : lundi 22 septembre 2014 - 17:39:15
Dernière modification le : jeudi 11 janvier 2018 - 06:22:24

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Aurélie Budin-Verneuil, Emmanuelle Maguin, Yanick Auffray, S Dusko Ehrlich, Vianney Pichereau. Transcriptional analysis of the cyclopropane fatty acid synthase gene of Lactococcus lactis MG1363 at low pH.. FEMS Microbiology Letters, Wiley-Blackwell, 2005, 250 (2), pp.189-94. 〈10.1016/j.femsle.2005.07.007〉. 〈hal-01067008〉

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