Transcriptional analysis of the cyclopropane fatty acid synthase gene of Lactococcus lactis MG1363 at low pH. - Université de Bretagne Occidentale Access content directly
Journal Articles FEMS Microbiology Letters Year : 2005

Transcriptional analysis of the cyclopropane fatty acid synthase gene of Lactococcus lactis MG1363 at low pH.

Abstract

Cyclopropane fatty acid synthase (cfa) catalyses the transfer of a methyl group from S-adenosylmethionine (SAM) to unsaturated fatty acids. Northern blot experiments demonstrated that the Lactococcus lactis MG1363 cfa gene is mainly expressed as a bicistronic transcript together with metK, the gene encoding SAM synthetase, and is highly induced by acidity. The cfa promoter was characterized by 5'-RACE PCR, and fused to beta-galactosidase by cloning into the pAK80 plasmid. This transcriptional fusion was highly induced by acidity (23-fold at pH 5) as well as during entry into the stationary phase (8-fold) in L. lactis. Interestingly, the cfa promoter expression is repressed in a L. lactis relA* mutant which accumulates (p)ppGpp, whereas its induction by acidity appeared independent of (p)ppGpp in L. lactis and in Escherichia coli.

Dates and versions

hal-01067008 , version 1 (22-09-2014)

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Aurélie Budin-Verneuil, Emmanuelle Maguin, Yanick Auffray, S Dusko Ehrlich, Vianney Pichereau. Transcriptional analysis of the cyclopropane fatty acid synthase gene of Lactococcus lactis MG1363 at low pH.. FEMS Microbiology Letters, 2005, 250 (2), pp.189-94. ⟨10.1016/j.femsle.2005.07.007⟩. ⟨hal-01067008⟩
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