Screening for the production of polyunsaturated fatty acids and cerebrosides in fungi - Université de Bretagne Occidentale Access content directly
Journal Articles Journal of Applied Microbiology Year : 2024

Screening for the production of polyunsaturated fatty acids and cerebrosides in fungi

Abstract

Abstract Aims To investigate fatty acid, including polyunsaturated fatty acids (PUFA), and cerebroside production of a large diversity of fungi from the Ascomycota, Basidiomycota, and Mucoromycota phyla. Methods and results Seventy-nine fungal strains were grown in Kavadia medium using a microcultivation system, i.e. Duetz microtiter plates. Following cultivation, fatty acid and cerebroside contents were analyzed by gas chromatography-flame ionization detection (GC-FID) and high performance thin-layer chromatography (HPTLC), respectively. Mucoromycota fungi appeared as the most promising candidates for omega-6 PUFA production. The best omega-6 producer, including γ-linolenic acid (GLA, 18:3n-6), was Mucor fragilis UBOCC-A109196 with a concentration of 647 mg L−1 total omega-6 PUFA (representing 35% of total fatty acids) and 225 mg L−1 GLA (representing 12% of total fatty acids). Arachidonic acid concentration (20:4n-6) was the highest in Mortierella alpina UBOCC-A-112046, reaching 255 mg L−1 and 18.56% of total fatty acids. Interestingly, several fungal strains were shown to produce omega-7 monounsaturated fatty acids. Indeed, Torulaspora delbrueckii strains accumulated palmitoleic acid (16:1n-7) up to 20% of total fatty acids, reaching 114 mg L−1 in T. delbrueckii UBOCC-A-214128, while C. elegans UBOCC-A-102008 produced mainly paullinic acid (20:1n-7) with concentrations up to 100 mg L−1. Concerning cerebroside production, HPTLC appeared as a relevant approach for their detection and quantification. Promising candidates belonging to the Mucoromycota phylum were found, especially in the Absidia genus with A. spinosa UBOCC-A-101332 as the best producer (12.7 mg L−1). Conclusions The present study highlighted PUFA and cerebroside production in a large diversity of fungi and the fact that members of the Mucoromycota phylum are good producers of PUFA as well as cerebrosides.
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Dates and versions

hal-04495046 , version 1 (08-03-2024)

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Laurie Calarnou, Estelle Vigouroux, Bertrand Thollas, Fabienne Le Grand, Jérôme Mounier. Screening for the production of polyunsaturated fatty acids and cerebrosides in fungi. Journal of Applied Microbiology, 2024, 135 (2), ⟨10.1093/jambio/lxae030⟩. ⟨hal-04495046⟩
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