Classical photo-induced fluorescence methods are conducted in two steps: a UV irradiation step in order to form a photo-induced compound followed by its fluorimetric determination. Automated flow injection methods are frequently used for these analyses. In this work, we propose a new method of direct laser photo-induced fluorescence analysis. This new method is based on direct irradiation of the analyte in a fluorimetric cell in order to form a photo-induced fluorescent compound and its direct fluorimetric detection during a short irradiation time. Irradiation is performed with a tuneable Nd:YAG laser to select the optimal excitation wavelength and to improve the specificity. It has been applied to the determination of bisphenol A, an endocrine disrupter compound that may be a potential contaminant for food. Irradiation of bisphenol A at 230 nm produces a photo-induced compound with a much higher fluorescence quantum yield and specific excitation/emission wavelengths. In tap water, the fluorescence of bisphenol A increases linearly versus its concentration and, its determination by direct laser photo-induced fluorescence permits to obtain a low limit of detection of 17 μg L(-1)