Pneumocystis jirovecii in the air surrounding patients with Pneumocystis pulmonary colonization.

Solène Le Gal; Laurence Pougnet; Céline Damiani; Emilie Fréalle; Paul Guéguen; Michèle Virmaux; Séverine Ansart; Sylvain Jaffuel; Francis Couturaud; Aurélien Delluc; Jean-Marie Tonnelier; Philippe Castellant; Yann Le Meur; Gaétan Le Floch; Anne Totet; Jean Menotti; Gilles Nevez

doi:10.1016/j.diagmicrobio.2015.01.004

Pneumocystis jirovecii in the air surrounding patients with Pneumocystis pulmonary colonization.

Solène Le Gal ^{1, 2} Laurence Pougnet ³ Céline Damiani ^{4, 5} Emilie Fréalle ⁶ Paul Guéguen ^{7, 8} Michèle Virmaux ¹ Séverine Ansart ^{9, 10} Sylvain Jaffuel ¹⁰ Francis Couturaud ^{11, 12} Aurélien Delluc ^{12, 11} Jean-Marie Tonnelier ¹³ Philippe Castellant ¹⁴ Yann Le Meur ¹⁵ Gaétan Le Floch ¹⁶ Anne Totet ¹⁷ Jean Menotti Gilles Nevez ^{1, 2}

1 LUBEM - Laboratoire Universitaire de Biodiversité et Ecologie Microbienne

2 Parasito - Myco - BREST - Laboratoire de Parasitologie et Mycologie

3 Hôpital d'Instruction des Armées Clermont Tonnerre

4 UMR-I 01 - AMIENS

5 Parasito - Myco - AMIENS - Laboratoire de Parasitologie et Mycologie

6 Parasito - Myco - LILLE - Laboratoire de Parasitologie et Mycologie

7 Génétique, génomique fonctionnelle et biotechnologies (UMR 1078)

8 Laboratoire de Génétique Moléculaire et d'Histocompatibilité [Brest]

9 LaTIM - Laboratoire de Traitement de l'Information Medicale

10 Service de médecine interne 2 et maladies infectieuses

11 DMIP - Brest - Département de Médecine Interne et Pneumologie [Brest]

12 GETBO - Groupe d'Etude de la Thrombose de Bretagne Occidentale

13 BREST - Réa Med - Service de Réanimation Médicale

14 ORPHY (EA 4324) - Optimisation des régulations physiologiques

15 EA2216 - Immunologie et Pathologie

16 IDPSP - Institut du droit public et de la science politique

17 Departments of Medical Parasitology and Mycology

Abstract : In this study, Pneumocystis jirovecii was detected and characterized in the air surrounding patients with Pneumocystis pulmonary colonization. Air samples were collected in the rooms of 10 colonized patients using Coriolis® μ air sampler at 1m and 5m from the patient's head. P. jirovecii DNA was amplified and genotyped in pulmonary and air samples at the mitochondrial large subunit ribosomal RNA gene. P. jirovecii DNA was detected in 5 of the 10 air samples collected at 1m and in 5 of the 10 other air samples collected at 5m. P. jirovecii genotyping was successful in 4 pairs or triplets of air and pulmonary samples. Full genotype matches were observed in 3 of the 4 pairs or triplets of air and pulmonary samples. These results provide original data supporting P. jirovecii exhalation from colonized patients and emphasize the risk of P. jirovecii nosocomial transmission from this patient population.

Keywords : Pneumocystis jirovecii colonization airborne transmission exhalation

Type de document :

Article dans une revue

Diagnostic Microbiology and Infectious Disease, Elsevier, 2015, 82 (2), pp.137-42. 〈10.1016/j.diagmicrobio.2015.01.004〉

Domaine :

Sciences du Vivant [q-bio]

Sciences du Vivant [q-bio] / Médecine humaine et pathologie

Liste complète des métadonnées

http://hal.univ-brest.fr/hal-01275093
Contributeur : Ghislaine Calvez <>
Soumis le : mardi 16 février 2016 - 17:00:24
Dernière modification le : lundi 26 mars 2018 - 15:25:06

Pneumocystis jirovecii in the air surrounding patients with Pneumocystis pulmonary colonization.

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