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Pneumocystis jirovecii in the air surrounding patients with Pneumocystis pulmonary colonization.

Solène Le Gal 1, 2 Laurence Pougnet 3 Céline Damiani 4, 5 Emilie Fréalle 6 Paul Guéguen 7, 8 Michèle Virmaux 1 Séverine Ansart 9, 10 Sylvain Jaffuel 10 Francis Couturaud 11, 12 Aurélien Delluc 12, 11 Jean-Marie Tonnelier 13 Philippe Castellant 14 Yann Le Meur 15 Gaétan Le Floch 16 Anne Totet 17 Jean Menotti Gilles Nevez 1, 2
1 LUBEM - Laboratoire Universitaire de Biodiversité et Ecologie Microbienne
2 Parasito - Myco - BREST - Laboratoire de Parasitologie et Mycologie
3 Hôpital d'Instruction des Armées Clermont Tonnerre
4 UMR-I 01 - AMIENS
5 Parasito - Myco - AMIENS - Laboratoire de Parasitologie et Mycologie
6 Parasito - Myco - LILLE - Laboratoire de Parasitologie et Mycologie
7 GGB - Génétique, génomique fonctionnelle et biotechnologies (UMR 1078)
8 Laboratoire de Génétique Moléculaire et d'Histocompatibilité [Brest]
9 LaTIM - Laboratoire de Traitement de l'Information Medicale
10 Service de médecine interne 2 et maladies infectieuses
11 DMIP - Brest - Département de Médecine Interne et Pneumologie [Brest]
12 GETBO - Groupe d'Etude de la Thrombose de Bretagne Occidentale
13 BREST - Réa Med - Service de Réanimation Médicale
14 ORPHY (EA 4324) - Optimisation des régulations physiologiques
15 EA2216 - Immunologie et Pathologie
16 IDPSP - Institut du droit public et de la science politique
17 Departments of Medical Parasitology and Mycology
Abstract : In this study, Pneumocystis jirovecii was detected and characterized in the air surrounding patients with Pneumocystis pulmonary colonization. Air samples were collected in the rooms of 10 colonized patients using Coriolis® μ air sampler at 1m and 5m from the patient's head. P. jirovecii DNA was amplified and genotyped in pulmonary and air samples at the mitochondrial large subunit ribosomal RNA gene. P. jirovecii DNA was detected in 5 of the 10 air samples collected at 1m and in 5 of the 10 other air samples collected at 5m. P. jirovecii genotyping was successful in 4 pairs or triplets of air and pulmonary samples. Full genotype matches were observed in 3 of the 4 pairs or triplets of air and pulmonary samples. These results provide original data supporting P. jirovecii exhalation from colonized patients and emphasize the risk of P. jirovecii nosocomial transmission from this patient population.
Keywords : Pneumocystis jirovecii colonization airborne transmission exhalation
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Journal articles
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https://hal.univ-brest.fr/hal-01275093
Contributor : Ghislaine Calvez <>
Submitted on : Tuesday, February 16, 2016 - 5:00:24 PM
Last modification on : Wednesday, June 24, 2020 - 4:18:11 PM

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UNIV-BREST | UNIV-RENNES1 | SSA | INSTITUT-TELECOM | IDPSP | GETBO | IMMUNO | UR1-DROIT | UNIV-RENNES | LUBEM | ORPHY | GGFB | LATIM | IBSAM

Citation

Solène Le Gal, Laurence Pougnet, Céline Damiani, Emilie Fréalle, Paul Guéguen, et al.. Pneumocystis jirovecii in the air surrounding patients with Pneumocystis pulmonary colonization.. Diagnostic Microbiology and Infectious Disease, Elsevier, 2015, 82 (2), pp.137-42. ⟨10.1016/j.diagmicrobio.2015.01.004⟩. ⟨hal-01275093⟩

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