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Journal articles
Insights into the respiratory tract microbiota of patients with cystic fibrosis during early Pseudomonas aeruginosa colonization.
Marlène Keravec
1
Jérôme Mounier
1
Emmanuel Prestat
2, 3
Sophie Vallet
4, 1
Janet K Jansson
5, 3
Gaëtan Burgaud
1
Sylvain Rosec
6
Stéphanie Gouriou
1
Gilles Rault
7
Emmanuel Coton
1
Georges Barbier
1
Geneviève Héry-Arnaud
1, 4, *
*
Corresponding author
Marlène Keravec 1
Author
Jérôme Mounier 1
Author
Emmanuel Prestat 2, 3
Author
Sophie Vallet 4, 1
Author
Janet K Jansson 5, 3
Author
Gaëtan Burgaud 1
Author
Sylvain Rosec 6
Author
Stéphanie Gouriou 1
Author
Gilles Rault 7
Author
Emmanuel Coton 1
Author
Georges Barbier 1
Author
Geneviève Héry-Arnaud 1, 4
Correspondent author
1
LUBEM - Laboratoire Universitaire de Biodiversité et Ecologie Microbienne (ESIAB - Parvis Blaise Pascal - Technopôle Brest-Iroise - 29280 Plouzané - France)
- UBO - Université de Brest : EA3882 (Université de Bretagne Occidentale - 3 Rue des Archives 29238, Brest - France)
2
Qiagen Marseille (Case 923 - 163 avenue de Luminy 13288 Marseille - Cedex 9 - France)
- Luminy Biotech Entreprises (France)
3
LBNL - Lawrence Berkeley National Laboratory [Berkeley] (1 Cyclotron Rd. MS 50A-1148, Berkeley, CA 94720 - United States)
4
Département de Bactériologie-Virologie (Hygiène Hospitalière et Parasitologie-Mycologie - 51 Rue Professeur Langevin 29200 Brest - France)
5
Biological Sciences Division (Richland, WA - United States)
- PNNL - Pacific Northwest National Laboratory (Richland, WA 99352 - United States)
6
CIC - Brest - Centre d'Investigation Clinique (CHU de la Cavale Blanche 29609 Brest - France)
- UBO - Université de Brest (Université de Bretagne Occidentale - 3 Rue des Archives 29238, Brest - France)
- INSERM - Institut National de la Santé et de la Recherche Médicale : CIC502 (101, rue de Tolbiac, 75013 Paris - France)
7
CRCM - Centre de Ressources et de Compétences de la Mucoviscidose (Presqu'île de Perharidy 29684 Roscoff cedex - France)
- Centre de Perharidy (France)
- Cellule de Coordination du Réseau Mucoviscidose (CECOREM) (France)
Abstract : Pseudomonas aeruginosa plays a major role in cystic fibrosis (CF) progression. Therefore, it is important to understand the initial steps of P. aeruginosa infection. The structure and dynamics of CF respiratory tract microbial communities during the early stages of P. aeruginosa colonization were characterized by pyrosequencing and cloning-sequencing. The respiratory microbiota showed high diversity, related to the young age of the CF cohort (mean age 10 years). Wide inter- and intra-individual variations were revealed. A common core microbiota of 5 phyla and 13 predominant genera was found, the majority of which were obligate anaerobes. A few genera were significantly more prevalent in patients never infected by P. aeruginosa. Persistence of an anaerobic core microbiota regardless of P. aeruginosa status suggests a major role of certain anaerobes in the pathophysiology of lung infections in CF. Some genera may be potential biomarkers of pulmonary infection state.
Document type :
Journal articles
Complete list of metadatas
https://hal.univ-brest.fr/hal-01272828
Contributor : Ghislaine Calvez <>
Submitted on : Thursday, February 11, 2016 - 2:19:35 PM
Last modification on : Thursday, November 29, 2018 - 4:08:48 PM
Contributor : Ghislaine Calvez <>
Submitted on : Thursday, February 11, 2016 - 2:19:35 PM
Last modification on : Thursday, November 29, 2018 - 4:08:48 PM
Links full text
Identifiers
- HAL Id : hal-01272828, version 1
- DOI : 10.1186/s40064-015-1207-0
- PUBMED : 26266076
- PUBMEDCENTRAL : PMC4529844
Collections
UNIV-BREST | LUBEM | IBSAM
Citation
Marlène Keravec, Jérôme Mounier, Emmanuel Prestat, Sophie Vallet, Janet K Jansson, et al.. Insights into the respiratory tract microbiota of patients with cystic fibrosis during early Pseudomonas aeruginosa colonization.. SpringerPlus, SpringerOpen, 2014, 4, pp.405. ⟨10.1186/s40064-015-1207-0⟩. ⟨hal-01272828⟩