Skip to Main content Skip to Navigation
New interface
Journal articles

Evidence of active methanogen communities in shallow sediments of the Sonora Margin cold seeps

Abstract : In the Sonora Margin cold seep ecosystems (Gulf of California), sediments underlying microbial mats harbor high biogenic methane concentrations, fuelling various microbial communities such as abundant lineages of Anaerobic Methanotrophs (ANME). However biodiversity, distribution and metabolism of the microorganisms producing this methane remain poorly understood. In this study, measurements of methanogenesis using radiolabelled dimethylamine, bicarbonate and acetate showed that biogenic methane production in these sediments was mainly dominated by methylotrophic methanogenesis, while the proportion of autotrophic methanogenesis increased with depth. Congruently, methane production and methanogenic Archaea were detected in culture enrichments amended with trimethylamine and bicarbonate. Analyses of DGGE fingerprinting and reverse-transcribed PCR amplified 16S rRNA sequences retrieved from these enrichments revealed the presence of active methylotrophic Methanococcoides burtonii relatives and several new autotrophic Methanogenium lineages confirming the co-occurrence of Methanosarcinales and Methanomicrobiales methanogens with abundant ANME populations in the sediments of the Sonora Margin cold seeps.
Complete list of metadata

Cited literature [70 references]  Display  Hide  Download
Contributor : Erwan Roussel Connect in order to contact the contributor
Submitted on : Thursday, April 23, 2015 - 12:18:23 PM
Last modification on : Friday, August 5, 2022 - 9:24:17 AM
Long-term archiving on: : Monday, September 14, 2015 - 12:27:03 PM


Vigneron 2015.pdf
Publisher files allowed on an open archive


Distributed under a Creative Commons Attribution - NonCommercial 4.0 International License




Adrien Vigneron, Stéphane L'Haridon, Anne Godfroy, Erwan Roussel, Barry A Cragg, et al.. Evidence of active methanogen communities in shallow sediments of the Sonora Margin cold seeps. Applied and Environmental Microbiology, 2015, 81 (10), pp.3451-3459. ⟨10.1128/AEM.00147-15⟩. ⟨hal-01144705⟩



Record views


Files downloads