Evidence of active methanogen communities in shallow sediments of the Sonora Margin cold seeps - Université de Bretagne Occidentale Access content directly
Journal Articles Applied and Environmental Microbiology Year : 2015

Evidence of active methanogen communities in shallow sediments of the Sonora Margin cold seeps

Abstract

In the Sonora Margin cold seep ecosystems (Gulf of California), sediments underlying microbial mats harbor high biogenic methane concentrations, fuelling various microbial communities such as abundant lineages of Anaerobic Methanotrophs (ANME). However biodiversity, distribution and metabolism of the microorganisms producing this methane remain poorly understood. In this study, measurements of methanogenesis using radiolabelled dimethylamine, bicarbonate and acetate showed that biogenic methane production in these sediments was mainly dominated by methylotrophic methanogenesis, while the proportion of autotrophic methanogenesis increased with depth. Congruently, methane production and methanogenic Archaea were detected in culture enrichments amended with trimethylamine and bicarbonate. Analyses of DGGE fingerprinting and reverse-transcribed PCR amplified 16S rRNA sequences retrieved from these enrichments revealed the presence of active methylotrophic Methanococcoides burtonii relatives and several new autotrophic Methanogenium lineages confirming the co-occurrence of Methanosarcinales and Methanomicrobiales methanogens with abundant ANME populations in the sediments of the Sonora Margin cold seeps.
Fichier principal
Vignette du fichier
Vigneron 2015.pdf (1.27 Mo) Télécharger le fichier
Origin : Publisher files allowed on an open archive
Loading...

Dates and versions

hal-01144705 , version 1 (23-04-2015)

Licence

Attribution - NonCommercial

Identifiers

Cite

Adrien Vigneron, Stéphane L'Haridon, Anne Godfroy, Erwan Roussel, Barry A Cragg, et al.. Evidence of active methanogen communities in shallow sediments of the Sonora Margin cold seeps. Applied and Environmental Microbiology, 2015, 81 (10), pp.3451-3459. ⟨10.1128/AEM.00147-15⟩. ⟨hal-01144705⟩
177 View
394 Download

Altmetric

Share

Gmail Facebook X LinkedIn More