NMR use to quantify phlorotannins: The case of Cystoseira tamariscifolia, a phloroglucinol-producing brown macroalga in Brittany (France)

Camille Jégou; Nelly Kervarec; Stéphane Cérantola; Isabelle Bihannic; Valérie Stiger-Pouvreau

doi:10.1016/j.talanta.2014.11.059

NMR use to quantify phlorotannins: The case of Cystoseira tamariscifolia, a phloroglucinol-producing brown macroalga in Brittany (France)

Camille Jégou ¹ Nelly Kervarec ² Stéphane Cérantola ² Isabelle Bihannic ³ Valérie Stiger-Pouvreau ^{3, *}

* Auteur correspondant

1 LUBEM - Laboratoire Universitaire de Biodiversité et Ecologie Microbienne

2 Service de Résonance Magnétique Nucléaire

3 LEMAR - Laboratoire des Sciences de l'Environnement Marin (LEMAR)

Abstract : Among the most renowned natural products from brown algae, phlorotannins are phloroglucinol polymers that have been extensively studied, both for their biotechnological potential and their interest in chemical ecology. The accurate quantification of these compounds is a key point to understand their role as mediators of chemical defense. In recent years, the Folin-Ciocalteu assay has remained a classic protocol for phlorotannin quantification, even though it frequently leads to over-estimations. Furthermore, the quantification of the whole pool of phlorotannins may not be relevant in ecological surveys. In this study, we propose a rapid 1H qNMR method for the quantification of phlorotannins. We identified phloroglucinol as the main phenolic compound produced by the brown macroalga Cystoseira tamariscifolia. This monomer was detected in vivo using 1H HR-MAS spectroscopy. We quantified this molecule through 1H qNMR experiments using TSP as internal standard. The results are discussed by comparison with a standard Folin-Ciocalteu assay performed on purified extracts. The accuracy and simplicity of qNMR makes this method a good candidate as a standard phlorotannin assay.

Keywords : Phlorotannin qNMR Phloroglucinol Folin-Ciocalteu HR-MAS Cystoseira tamariscifolia

Type de document :

Article dans une revue

Talanta, Elsevier, 2015, 135, pp.1-6. 〈10.1016/j.talanta.2014.11.059〉

Domaine :

Sciences du Vivant [q-bio]

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http://hal.univ-brest.fr/hal-01111281
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Soumis le : vendredi 30 janvier 2015 - 08:53:35
Dernière modification le : jeudi 13 septembre 2018 - 14:56:02

NMR use to quantify phlorotannins: The case of Cystoseira tamariscifolia, a phloroglucinol-producing brown macroalga in Brittany (France)

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NMR use to quantify phlorotannins: The case of Cystoseira tamariscifolia, a phloroglucinol-producing brown macroalga in Brittany (France)

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