Exploring the hologenome concept in marine bivalvia: haemolymph microbiota as a pertinent source of probiotics for aquaculture.

Florie Desriac 1 Patrick Le Chevalier 1 Benjamin Brillet 1 Ivan Leguerinel 1 Benoît Thuillier 2 Christine Paillard 3 Yannick Fleury 1, *
* Auteur correspondant
1 LUBEM - Laboratoire Universitaire de Biodiversité et Ecologie Microbienne
2 Inst. dept. d'analyses de conseil et d'expertise en hygiene alimentaire eau et environ. et sante
3 LEMAR - Laboratoire des Sciences de l'Environnement Marin (LEMAR)
Abstract : Haemolymph-associated microbiota of marine bivalves was explored for antibacterial activity against important aquaculture pathogens. A collection of 843 strains were cultured from the haemolymph of four bivalve species (Crassostrea gigas, Mytilus edulis, Pecten maximus and Tapes rhomboides) collected by deep-sea diving in the Glenan Archipelago (France). Cell-free culture supernatants were investigated for antibacterial activity using the well-diffusion assay. About 3% of haemolymph-associated cultivable bacteria displayed antibacterial activity toward Gram-negative pathogens. Among the active bacteria, Pseudoalteromonas strains exhibited the highest antibacterial activity. The cell-free culture supernatant of one of them, named hCg-51, was able to inhibit the growth of bacterial pathogens even after drastic dilution (1 : 1024). Hemocyte survival was not significantly altered in the presence of the haemolymph-associated strains assayed. Moreover, a dose-dependent beneficial effect on hemocyte survival rates was observed with the hCg-51 strain. These results suggest that haemolymph microbiota may participate in bivalve protection and therefore confer a health benefit on the host. As a result, the results highlight bivalve haemolymph microbiota as a promising novel source for aquaculture probiotics. This work also gives a first insight into the contribution of the haemolymph-associated microbiota as part of the bivalve 'hologenome'.
Type de document :
Article dans une revue
FEMS Microbiology Letters, Wiley-Blackwell, 2014, 350 (1), pp.107-16. 〈10.1111/1574-6968.12308〉
Liste complète des métadonnées
http://hal.univ-brest.fr/hal-00948603
Contributeur : Adminhal Univbrestbu <>
Soumis le : mardi 18 février 2014 - 14:59:23
Dernière modification le : jeudi 11 janvier 2018 - 06:22:24

Lien texte intégral

openaccess

Identifiants

Collections

IUEM | LEMAR | UNIV-BREST | LUBEM | THESES_IUEM | CMM

Citation

Florie Desriac, Patrick Le Chevalier, Benjamin Brillet, Ivan Leguerinel, Benoît Thuillier, et al.. Exploring the hologenome concept in marine bivalvia: haemolymph microbiota as a pertinent source of probiotics for aquaculture.. FEMS Microbiology Letters, Wiley-Blackwell, 2014, 350 (1), pp.107-16. 〈10.1111/1574-6968.12308〉. 〈hal-00948603〉

Exporter

BibTeX TEI DC DCterms EndNote

Partager

Métriques

Consultations de la notice

294

Contact

support.ccsd.cnrs.fr
hal.support@ccsd.cnrs.fr
Logo CCSD