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Exploring the hologenome concept in marine bivalvia: haemolymph microbiota as a pertinent source of probiotics for aquaculture.

Abstract : Haemolymph-associated microbiota of marine bivalves was explored for antibacterial activity against important aquaculture pathogens. A collection of 843 strains were cultured from the haemolymph of four bivalve species (Crassostrea gigas, Mytilus edulis, Pecten maximus and Tapes rhomboides) collected by deep-sea diving in the Glenan Archipelago (France). Cell-free culture supernatants were investigated for antibacterial activity using the well-diffusion assay. About 3% of haemolymph-associated cultivable bacteria displayed antibacterial activity toward Gram-negative pathogens. Among the active bacteria, Pseudoalteromonas strains exhibited the highest antibacterial activity. The cell-free culture supernatant of one of them, named hCg-51, was able to inhibit the growth of bacterial pathogens even after drastic dilution (1 : 1024). Hemocyte survival was not significantly altered in the presence of the haemolymph-associated strains assayed. Moreover, a dose-dependent beneficial effect on hemocyte survival rates was observed with the hCg-51 strain. These results suggest that haemolymph microbiota may participate in bivalve protection and therefore confer a health benefit on the host. As a result, the results highlight bivalve haemolymph microbiota as a promising novel source for aquaculture probiotics. This work also gives a first insight into the contribution of the haemolymph-associated microbiota as part of the bivalve 'hologenome'.
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Submitted on : Tuesday, February 18, 2014 - 2:59:23 PM
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Florie Desriac, Patrick Le Chevalier, Benjamin Brillet, Ivan Leguerinel, Benoît Thuillier, et al.. Exploring the hologenome concept in marine bivalvia: haemolymph microbiota as a pertinent source of probiotics for aquaculture.. FEMS Microbiology Letters, Wiley-Blackwell, 2014, 350 (1), pp.107-16. ⟨10.1111/1574-6968.12308⟩. ⟨hal-00948603⟩

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