Multiplex PCR sets of novel microsatellite loci for the great scallop Pecten maximus and their application in parentage assignment

Romain Morvezen 1 Florence Cornette 2 Grégory Charrier 3 Bruno Guinand 4 Sylvie Lapegue 2 Pierre Boudry 5 Jean Laroche 1, *
* Auteur correspondant
1 LEMAR - Laboratoire des Sciences de l'Environnement Marin (LEMAR)
2 LGP - Laboratoire de Génétique et Pathologie
3 Department of Biological and Environmental Sciences-Tjärnö
4 ISEM - Institut des Sciences de l'Evolution de Montpellier
5 LPI - Laboratoire de Physiologie des Invertébrés [Plouzané]
Abstract : We report the isolation, development and multiplex optimisation of 12 new microsatellite loci for the great scallop, Pecten maximus. Diversity was moderate to high, with number of alleles ranging from 4 to 20 and observed heterozygosity between 0.28 and 0.88. Progeny produced in a commercial hatchery was used to test locus power for parentage assignment. The percentage of offspring that was unambiguously assigned to a unique pair of parents was 97% (software package CERVUS-COLONY). Parentage assignment revealed that 22% of the studied progeny resulted from unplanned crosses. Effective population size of the study progeny was also estimated. Our study illustrates the power of microsatellites for the genetic monitoring of hatchery-produced great scallops.
Keywords : Microsatellites Polymerase chain reaction Parentage Assignment Bivalve mollusc
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Article dans une revue
Aquatic Living Resources, EDP Sciences, 2013, 26 (3), pp.207-213. 〈10.1051/alr/2013052〉
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Romain Morvezen, Florence Cornette, Grégory Charrier, Bruno Guinand, Sylvie Lapegue, et al.. Multiplex PCR sets of novel microsatellite loci for the great scallop Pecten maximus and their application in parentage assignment. Aquatic Living Resources, EDP Sciences, 2013, 26 (3), pp.207-213. 〈10.1051/alr/2013052〉. 〈hal-00946729〉

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