Skip to Main content Skip to Navigation
Journal articles

Measuring the scattering coefficient of turbid media from two-photon microscopy

Abstract : In this paper, we propose a new and simple method based on two-photon excitation fluorescence (TPEF) microscopy to measure the scattering coefficient μs of thick turbid media. We show, from Monte Carlo simulations, that μs can be derived from the axial profile of the ratio of the TPEF signals epi-collected by the confocal and the non-descanned ports of a scanning microscope, independently of the anisotropy factor g and of the absorption coefficient μa of the medium. The method is validated experimentally on tissue-mimicking optical phantoms, and is shown to have potential for imaging the scattering coefficient of heterogeneous media
Document type :
Journal articles
Complete list of metadatas

https://hal.univ-brest.fr/hal-00907898
Contributor : Matthieu Dubreuil <>
Submitted on : Friday, November 22, 2013 - 8:43:19 AM
Last modification on : Thursday, February 21, 2019 - 10:34:08 AM
Document(s) archivé(s) le : Sunday, February 23, 2014 - 4:22:51 AM

File

Measuring_the_scattering_coeff...
Publisher files allowed on an open archive

Identifiers

Citation

David Sevrain, Matthieu Dubreuil, Aymeric Leray, Christophe Odin, Yann Le Grand. Measuring the scattering coefficient of turbid media from two-photon microscopy. Optics Express, Optical Society of America, 2013, 21 (21), pp.25221. ⟨10.1364/OE.21.025221⟩. ⟨hal-00907898⟩

Share

Metrics

Record views

391

Files downloads

841