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Measuring the scattering coefficient of turbid media from two-photon microscopy

Abstract : In this paper, we propose a new and simple method based on two-photon excitation fluorescence (TPEF) microscopy to measure the scattering coefficient μs of thick turbid media. We show, from Monte Carlo simulations, that μs can be derived from the axial profile of the ratio of the TPEF signals epi-collected by the confocal and the non-descanned ports of a scanning microscope, independently of the anisotropy factor g and of the absorption coefficient μa of the medium. The method is validated experimentally on tissue-mimicking optical phantoms, and is shown to have potential for imaging the scattering coefficient of heterogeneous media
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Contributor : Matthieu Dubreuil Connect in order to contact the contributor
Submitted on : Friday, November 22, 2013 - 8:43:19 AM
Last modification on : Thursday, August 4, 2022 - 5:17:39 PM
Long-term archiving on: : Sunday, February 23, 2014 - 4:22:51 AM


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David Sevrain, Matthieu Dubreuil, Aymeric Leray, Christophe Odin, Yann Le Grand. Measuring the scattering coefficient of turbid media from two-photon microscopy. Optics Express, 2013, 21 (21), pp.25221. ⟨10.1364/OE.21.025221⟩. ⟨hal-00907898⟩



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