Novel inhibition of archaeal family-D DNA polymerase by uracil.

Tomas T Richardson 1 Louise Gilroy 1 Yoshizumi Ishino 2 Bernard A Connolly 1, * Ghislaine Henneke 3, *
* Auteur correspondant
1 Institute for Cell and Molecular Biosciences
2 Department of Genetic Resources Technology, Kyushu University
3 LM2E - Laboratoire de microbiologie des environnements extrêmophiles
Abstract : Archaeal family-D DNA polymerase is inhibited by the presence of uracil in DNA template strands. When the enzyme encounters uracil, following three parameters change: DNA binding increases roughly 2-fold, the rate of polymerization slows by a factor of ≈ 5 and 3'-5' proof-reading exonuclease activity is stimulated by a factor of ≈ 2. Together these changes result in a significant decrease in polymerization activity and a reduction in net DNA synthesis. Pol D appears to interact with template strand uracil irrespective of its distance ahead of the replication fork. Polymerization does not stop at a defined location relative to uracil, rather a general decrease in DNA synthesis is observed. 'Trans' inhibition, the slowing of Pol D by uracil on a DNA strand not being replicated is also observed. It is proposed that Pol D is able to interact with uracil by looping out the single-stranded template, allowing simultaneous contact of both the base and the primer-template junction to give a polymerase-DNA complex with diminished extension ability.
Keywords : CELL NUCLEAR ANTIGEN X-RAY CRYSTALLOGRAPHY IRON-SULFUR CLUSTER REPLICATION FORK PYROCOCCUS-ABYSSI HYPERTHERMOPHILIC ARCHAEON DEAMINATED BASES RECOGNITION REPAIR FURIOSUS
Type de document :
Article dans une revue
Nucleic Acids Research, Oxford University Press, 2013, 41 (7), pp.4207-18. 〈10.1093/nar/gkt083〉
Liste complète des métadonnées

Littérature citée [39 références]  Voir  Masquer  Télécharger
http://hal.univ-brest.fr/hal-00834323
Contributeur : Adminhal Univbrestbu <>
Soumis le : vendredi 14 juin 2013 - 16:44:53
Dernière modification le : jeudi 11 janvier 2018 - 06:16:39
Document(s) archivé(s) le : dimanche 15 septembre 2013 - 04:12:09

Fichier

Nucl._Acids_Res.-2013-Richards...
Fichiers éditeurs autorisés sur une archive ouverte

Identifiants

Collections

IUEM | UNIV-BREST | LM2E_UBO | THESES_IUEM | CMM

Citation

Tomas T Richardson, Louise Gilroy, Yoshizumi Ishino, Bernard A Connolly, Ghislaine Henneke. Novel inhibition of archaeal family-D DNA polymerase by uracil.. Nucleic Acids Research, Oxford University Press, 2013, 41 (7), pp.4207-18. 〈10.1093/nar/gkt083〉. 〈hal-00834323〉

Exporter

BibTeX TEI DC DCterms EndNote

Partager

Métriques

Consultations de la notice

445

Téléchargements de fichiers

262

Contact

support.ccsd.cnrs.fr
hal.support@ccsd.cnrs.fr
Logo CCSD