Differential expression of genes involved in immunity and biomineralization during Brown Ring Disease development and shell repair in the Manila clam, Ruditapes philippinarum.

Fanny Jeffroy; Franck Brulle; Christine Paillard

doi:10.1016/j.jip.2013.03.001

Differential expression of genes involved in immunity and biomineralization during Brown Ring Disease development and shell repair in the Manila clam, Ruditapes philippinarum.

Fanny Jeffroy ¹ Franck Brulle ¹ Christine Paillard ^{1, *}

* Auteur correspondant

1 LEMAR - Laboratoire des Sciences de l'Environnement Marin (LEMAR)

Abstract : Severe drop in Manila clams production in French aquacultured fields since the end of the 1980's is associated to Brown Ring Disease (BRD). This disease, caused by the bacteria Vibrio tapetis, is characterized by specific symptoms on the inner face of the shell. Diseased animals develop conchiolin deposit to enrobe bacteria and form new calcified layers on the shell. Suppression subtractive hybridization was performed to identify genes differentially expressed during the early interaction of V. tapetis and Ruditapes philippinarum. Results revealed 301 unique genes differentially expressed during V. tapetis challenge. Several candidates involved in immune and biomineralization processes were selected from libraries. Transcriptional expression of selected candidates was determined in hemolymph and mantle tissues and revealed spatial and temporal variations. At 56days after infection, when clams were in phase of shell repair, transcripts of galectin and ferritin in hemocytes showed higher expression. Ca-like and serpin transcripts were specifically expressed in mantle and could contribute to defense against BRD.

Keywords : Brown Ring Disease Gene expression Manila clam Mantle tissue SSH Vibrio tapetis

Type de document :

Article dans une revue

Journal of Invertebrate Pathology, Elsevier, 2013, 113 (2), pp.129-136. 〈10.1016/j.jip.2013.03.001〉

Domaine :

Sciences de l'environnement

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Soumis le : jeudi 25 avril 2013 - 09:13:01
Dernière modification le : jeudi 11 janvier 2018 - 06:22:24

Differential expression of genes involved in immunity and biomineralization during Brown Ring Disease development and shell repair in the Manila clam, Ruditapes philippinarum.

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Differential expression of genes involved in immunity and biomineralization during Brown Ring Disease development and shell repair in the Manila clam, Ruditapes philippinarum.

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