Evaluation of chromosomal damage by flow cytometry in turbot (Scophthalmus maximus L.) exposed to fuel oil.

Christelle Goanvec 1 Michaël Theron 1 Elisabeth Poirier Stéphane Le Floch 2 Jean Laroche 3 Liliane Nonnotte Guy Nonnotte
1 ORPHY (EA 4324) - Optimisation des régulations physiologiques
2 Cedre - Centre de documentation de recherche et d'expérimentations sur les pollutions accidentelles des eaux
3 LEMAR - Laboratoire des Sciences de l'Environnement Marin (LEMAR)
Abstract : Flatfishes, turbots (Scophthalmus maximus), were injected intraperitoneally with two doses of fuel oil number 2. Biliary metabolites were evaluated by fixed fluorescence to verify the efficiency of intoxication. Ethoxyresorufin-O-deethylase (EROD) activity was compared with chromosomal damage measured by flow cytometry. The analysis of biliary metabolites showed a good dose-response relation and constitutes a clear reference for the subsequent measurements. Comparing flow cytometry and EROD results, a shorter delay of response for EROD activity was obtained, but chromosomal damage was significant only after one week. The persistence of the EROD response was shorter, while the genotoxic signal still persisted after one month. The measurement of chromosomal damage allowed a good differentiation between the two tested doses. In the case of EROD activity, the results were less clear. The results suggest that within a few weeks after exposure to fuel oil number 2, the measurements of chromosomal damage by flow cytometry can be used to detect a dose-dependent genotoxic response in fish.
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Article dans une revue
Biomarkers, Taylor & Francis, 2004, 9 (6), pp.435-46. 〈10.1080/13547500400027001〉
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http://hal.univ-brest.fr/hal-00753922
Contributeur : François Guerrero <>
Soumis le : lundi 19 novembre 2012 - 21:55:20
Dernière modification le : jeudi 11 janvier 2018 - 06:22:24

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Christelle Goanvec, Michaël Theron, Elisabeth Poirier, Stéphane Le Floch, Jean Laroche, et al.. Evaluation of chromosomal damage by flow cytometry in turbot (Scophthalmus maximus L.) exposed to fuel oil.. Biomarkers, Taylor & Francis, 2004, 9 (6), pp.435-46. 〈10.1080/13547500400027001〉. 〈hal-00753922〉

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