Evaluation of chromosomal damage by flow cytometry in turbot (Scophthalmus maximus L.) exposed to fuel oil. - Université de Bretagne Occidentale Accéder directement au contenu
Article Dans Une Revue Biomarkers Année : 2004

Evaluation of chromosomal damage by flow cytometry in turbot (Scophthalmus maximus L.) exposed to fuel oil.

Résumé

Flatfishes, turbots (Scophthalmus maximus), were injected intraperitoneally with two doses of fuel oil number 2. Biliary metabolites were evaluated by fixed fluorescence to verify the efficiency of intoxication. Ethoxyresorufin-O-deethylase (EROD) activity was compared with chromosomal damage measured by flow cytometry. The analysis of biliary metabolites showed a good dose-response relation and constitutes a clear reference for the subsequent measurements. Comparing flow cytometry and EROD results, a shorter delay of response for EROD activity was obtained, but chromosomal damage was significant only after one week. The persistence of the EROD response was shorter, while the genotoxic signal still persisted after one month. The measurement of chromosomal damage allowed a good differentiation between the two tested doses. In the case of EROD activity, the results were less clear. The results suggest that within a few weeks after exposure to fuel oil number 2, the measurements of chromosomal damage by flow cytometry can be used to detect a dose-dependent genotoxic response in fish.
Fichier non déposé

Dates et versions

hal-00753922 , version 1 (19-11-2012)

Identifiants

Citer

Christelle Goanvec, Michaël Theron, Elisabeth Poirier, Stéphane Le Floch, Jean Laroche, et al.. Evaluation of chromosomal damage by flow cytometry in turbot (Scophthalmus maximus L.) exposed to fuel oil.. Biomarkers, 2004, 9 (6), pp.435-46. ⟨10.1080/13547500400027001⟩. ⟨hal-00753922⟩
167 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More