31P nuclear magnetic resonance measurements of phosphate metabolites and intracellular pH in turbot Psetta maxima red blood cells using a novel flow method. - Université de Bretagne Occidentale Access content directly
Journal Articles Journal of Fish Biology Year : 2009

31P nuclear magnetic resonance measurements of phosphate metabolites and intracellular pH in turbot Psetta maxima red blood cells using a novel flow method.

M. T. Thebault
  • Function : Author

Abstract

A method for oxygenating and mixing suspensions of turbot Psetta maxima red blood cells (RBC) was tested in (31)P nuclear magnetic resonance (NMR) spectroscopy. In normoxia, the levels of inorganic phosphate (Pi) and nucleoside triphosphates (NTP) were stable up to 140 min and intracellular pH (pHi) was maintained and decreased oxygen partial pressure (P(O ( 2) )) from 30 to 15 and 600 Pa produced a significant fall in the intensity of NTP resonance, balanced by an increase in the Pi signal. Treatment of RBC with 0. 5 M isoproterenol during hypoxia exposure did not affect the pattern of changes in NTP or pHi induced by hypoxia and the effect was manifest only on Pi levels.

Dates and versions

hal-00750436 , version 1 (09-11-2012)

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M. T. Thebault, K. Pichavant, N. Kervarec. 31P nuclear magnetic resonance measurements of phosphate metabolites and intracellular pH in turbot Psetta maxima red blood cells using a novel flow method.. Journal of Fish Biology, 2009, 75 (3), pp.747-54. ⟨10.1111/j.1095-8649.2009.02351.x⟩. ⟨hal-00750436⟩
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