Effect of training frequency on endothelium-dependent vasorelaxation in rats.

Erwan Heylen François Guerrero 1 Jacques Mansourati 1 Michaël Theron 1 Sanéo Thioub Bernard Saïag
1 ORPHY (EA 4324) - Optimisation des régulations physiologiques
Abstract : BACKGROUND: Moderate physical activity enhances endothelium-dependent vasorelaxation. Whether the frequency of exercise affects endothelial function is unclear. The purpose of this study was to investigate the effects of various frequencies of training on endothelium-dependent vasorelaxation. DESIGN: Male Wistar rats were trained for 8 weeks on a treadmill at various frequencies [1 (Ex1), 3 (Ex3) or 5 days/week (Ex5)] and compared with age-matched sedentary animals (SED). A control group allowed us to assess endothelial function before the exercise protocol. Rings of thoracic aorta were precontracted with phenylephrine. RESULTS: Endothelium-independent relaxation elicited by sodium nitroprusside was similar in all groups. The maximal response elicited by acetylcholine (ACh) was not different between groups, whereas pD2 values (-logEC50, EC50 being the concentration of ACh that elicited 50% of the maximal response) significantly correlated with frequency of training. nitro-L-arginine methyl ester (L-NAME) reduced the relaxation elicited by 10(-7) mol/l ACh or higher in control and all trained groups, and by 10(-6) mol/l ACh or higher in SED group. Indomethacin inhibited the vasodilating response to 10(-7) mol/l ACh or higher in control, SED and Ex1 groups, and to 10(-8) mol/l or more in Ex3 and Ex5 animals. Tetraethylammonium attenuated the response to 10(-6) mol/l ACh or higher in control and SED groups and to 10(-7) mol/l or more in all trained animals. CONCLUSION: This data suggest that decreased ACh-induced vasorelaxation after physical inactivity may result from impairment of endothelial nitric oxide synthase, prostacyclin and endothelium-derived hyperpolarizing factor pathways. This effect is prevented by training in a frequency-dependent manner.
Keywords : rat exercise training endothelium aorta acetylcholine NO COX EDHF
Type de document :
Article dans une revue
European Journal of Cardiovascular Prevention and Rehabilitation, SAGE Publications, 2008, 15 (1), pp.52-8. 〈10.1097/HJR.0b013e3282f17baa〉
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Contributeur : François Guerrero <>
Soumis le : jeudi 18 octobre 2012 - 22:51:25
Dernière modification le : jeudi 13 septembre 2018 - 15:17:13

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Erwan Heylen, François Guerrero, Jacques Mansourati, Michaël Theron, Sanéo Thioub, et al.. Effect of training frequency on endothelium-dependent vasorelaxation in rats.. European Journal of Cardiovascular Prevention and Rehabilitation, SAGE Publications, 2008, 15 (1), pp.52-8. 〈10.1097/HJR.0b013e3282f17baa〉. 〈hal-00743423〉

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