Assessment of activated protein C resistance using a new and rapid venom-based test: STA Staclot APC-R.

Emmanuel Oger 1 Christophe Leroyer 1, 2 Bernard Mercier 3 Patrick Van Dreden Luc Bressollette 1, 2 Luc De Saint-Martin 2 Emmanuelle Le Moigne 2 Marie-Thérèse Blouch 4 N. Thuillier J. Amiral Claude Ferec 3 Jean-François Abgrall 4 Dominique Mottier 1
1 GETBO - Groupe d'Etude de la Thrombose de Bretagne Occidentale
2 DMIP - Brest - Département de Médecine Interne et Pneumologie [Brest]
3 Génétique moléculaire et génétique épidémiologique
4 CHU-Brest-Hemato - CHRU Brest - Service d'Hématologie
Abstract : Activated protein C (APC) resistance is related to a single point mutation in the factor V gene (FV:Q506) and appears to be the most common inherited risk factor for venous thromboembolism. A reliable screening test is therefore useful. We aimed to evaluate a new APC resistance test, on the basis of the procoagulant activity present in one snake venom of a crotalidae family: STA Staclot APC-R. We studied 36 consecutive patients with an acute deep venous thrombosis (DVT) confirmed by compression ultrasonography and carrying the FV:Q506 allele, assessed by DNA analysis, 103 of their family members and 35 consecutive patients with a proven DVT but who did not carry the FV:Q506 allele. Blood samples were collected within 24 h of admission for the DVT cases and on the day of medical registration for the family members. Tests were performed blind. The STA Staclot APC-R test, using a cut-off value of 0.80, had an overall sensitivity of 100% (95% CI, 95-100) and a specificity of 98.8% (95% CI, 92.0-99.6). An acute thrombosis process did not influence the performance of the test. We conclude that this test is easy and rapid to perform in every day practice and fulfills the criteria for a screening test.
Type de document :
Article dans une revue
Blood Coagulation and Fibrinolysis, Lippincott, Williams & Wilkins, 1998, 9 (4), pp.355-9
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https://hal.univ-brest.fr/hal-00722368
Contributeur : Ghislaine Calvez <>
Soumis le : mercredi 1 août 2012 - 13:51:50
Dernière modification le : jeudi 29 novembre 2018 - 16:09:32

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  • HAL Id : hal-00722368, version 1
  • PUBMED : 9690807

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GETBO | UNIV-BREST | IBSAM

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Emmanuel Oger, Christophe Leroyer, Bernard Mercier, Patrick Van Dreden, Luc Bressollette, et al.. Assessment of activated protein C resistance using a new and rapid venom-based test: STA Staclot APC-R.. Blood Coagulation and Fibrinolysis, Lippincott, Williams & Wilkins, 1998, 9 (4), pp.355-9. 〈hal-00722368〉

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