HAL will be down for maintenance from Friday, June 10 at 4pm through Monday, June 13 at 9am. More information
Skip to Main content Skip to Navigation
Journal articles

Comparison of cryopreservation methods for the long term storage of the marine diatom Haslea ostrearia (simonsen)

Abstract : Long term maintenance of microalgal strains by serial subculturing is often expensive and time-consuming. Alternative methods, such as cryopreservation, present several benefits and thus seem more relevant. Our study aimed at comparing two cryopreservation procedures applied to the marine diatom Haslea ostrearia (Simonsen): (1) a two-step freezing method in liquid media using 5%, 10% and 20% MeOH, Me2SO or Glycerol, and (2) an immobilization-dehydration method consisting in an algal cell entrapped in 0.7 M sucrose dehydrated and air-flow desiccated calcium alginate beads before "direct" or "two-step" freezing. Our results showed that the cryopreservation of H. ostrearia was feasible. With the two-step freezing protocol only Me2SO maintained cell viability without contamination but the low percentage of viability (<10%) prevents its use. Conversely, the immobilization-dehydration methods tested in this study were effective. Average viability of 57% and 77% were obtained with the "direct" and the "two step" cooling assays respectively, ensuring preservation of the genetic traits of H. ostrearia.
Document type :
Journal articles
Complete list of metadata

https://hal.univ-brest.fr/hal-00721494
Contributor : Adminhal Univbrestbu Connect in order to contact the contributor
Submitted on : Friday, July 27, 2012 - 4:07:50 PM
Last modification on : Tuesday, May 10, 2022 - 3:25:39 PM

Identifiers

Citation

Anaelle Tanniou, Vincent Turpin, Thierry Lebeau. Comparison of cryopreservation methods for the long term storage of the marine diatom Haslea ostrearia (simonsen). Cryobiology, Elsevier, 2012, 65 (1), pp.45-50. ⟨10.1016/j.cryobiol.2012.03.011⟩. ⟨hal-00721494⟩

Share

Metrics

Record views

95