 |
Journal articles
Response of the Pacific oyster Crassostrea gigas, Thunberg 1793, to pesticide exposure under experimental conditions
Abstract : Pesticide run-off into the ocean represents a potential threat to marine organisms, especially bivalves living in coastal environments. However, little is known about the effects of environmentally relevant concentrations of pesticides at the individual level. In this study, the suppression subtractive hybridisation technique was used to discover the main physiological function affected by a cocktail of three pesticides (lindane, metolachlor and carbofuran) in the Pacific oyster Crassostrea gigas. Two oyster populations exposed to different pollution levels in the wild were investigated. The pesticide concentrations used to induce stress were close to those found in the wild. In a time course experiment, the expression of three genes implicated in iron metabolism and oxidative stress as well as that of two ubiquitous stress proteins was examined. No clear regulation of gene or protein expression was found, potentially due to a low-dose effect. However, we detected a strong site-and organ-specific response to the pesticides. This study thus (1) provides insight into bivalve responses to pesticide pollution at the level of the transcriptome, which is the first level of response for organisms facing pollution, and (2) raises interesting questions concerning the importance of the sites and organs studied in the toxicogenomic field.
Cited literature [51 references]
https://hal.univ-brest.fr/hal-00669935
Contributor : Catherine Bertignac Connect in order to contact the contributor
Submitted on : Monday, January 11, 2016 - 12:29:34 PM
Last modification on : Tuesday, May 10, 2022 - 3:25:39 PM
Long-term archiving on: : Tuesday, April 12, 2016 - 11:16:03 AM
Contributor : Catherine Bertignac Connect in order to contact the contributor
Submitted on : Monday, January 11, 2016 - 12:29:34 PM
Last modification on : Tuesday, May 10, 2022 - 3:25:39 PM
Long-term archiving on: : Tuesday, April 12, 2016 - 11:16:03 AM
File
4010.full.pdf
Publisher files allowed on an open archive