A Functional Study of Transforming Growth Factor-Beta from the Gonad of Pacific Oyster Crassostrea gigas

Charlotte Corporeau 1, 2, * Agnès Groisillier 3 Alexandra Jeudy 3 Tristan Barbeyron 3 Elodie Fleury 4 Caroline Fabioux 5 Mirjam Czjzek 3 Arnaud Huvet 4
* Auteur correspondant
1 LPI - Laboratoire de Physiologie des Invertébrés
2 PE2M - Physiologie et Ecophysiologie des Mollusques Marins
3 UMR7139 - Végétaux marins et biomolécules
4 PE2M - Physiologie et Ecophysiologie des Mollusques Marins
5 LEMAR - Laboratoire des Sciences de l'Environnement Marin (LEMAR)
Abstract : The transforming growth factor (TGF)-beta superfamily is a group of important growth factors involved in multiple processes such as differentiation, cell proliferation, apoptosis and cellular growth. In the Pacific oyster Crassostrea gigas, the oyster gonadal (og) TGF-beta gene was recently characterized through genome-wide expression profiling of oyster lines selected to be resistant or susceptible to summer mortality. Og TGF-beta appeared specifically expressed in the gonad to reach a maximum when gonads are fully mature, which singularly contrasts with the pleiotropic roles commonly ascribed to most TGF-beta family members. The function of og TGF-beta protein in oysters is unknown, and defining its role remains challenging. In this study, we develop a rapid bacterial production system to obtain recombinant og TGF-beta protein, and we demonstrate that og TGF-beta is processed by furin to a mature form of the protein. This mature form can be detected in vivo in the gonad. Functional inhibition of mature og TGF-beta in the gonad was conducted by inactivation of the protein using injection of antibodies. We show that inhibition of og TGF-beta function tends to reduce gonadic area. We conclude that mature og TGF-beta probably functions as an activator of germ cells development in oyster.
Keywords : Transforming growth factor-beta Bacterial expression In vivo antibody inhibition Reproduction Crassostrea gigas Marine bivalve
Type de document :
Article dans une revue
Marine Biotechnology, Springer Verlag, 2011, 13 (5), pp.971-980. 〈10.1007/s10126-010-9361-4〉
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Contributeur : Catherine Bertignac <>
Soumis le : lundi 13 février 2012 - 15:20:09
Dernière modification le : mardi 5 juin 2018 - 10:14:30

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Charlotte Corporeau, Agnès Groisillier, Alexandra Jeudy, Tristan Barbeyron, Elodie Fleury, et al.. A Functional Study of Transforming Growth Factor-Beta from the Gonad of Pacific Oyster Crassostrea gigas. Marine Biotechnology, Springer Verlag, 2011, 13 (5), pp.971-980. 〈10.1007/s10126-010-9361-4〉. 〈hal-00669618〉

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