Molecular identification and expression of the phosphoglucomutase (PGM) gene from the Pacific oyster Crassostrea gigas

Arnaud Tanguy; Isabelle Boutet; Pierre Boudry; Lionel Degremont; Jean Laroche; Dario Moraga

doi:10.1016/j.gene.2006.06.005

Molecular identification and expression of the phosphoglucomutase (PGM) gene from the Pacific oyster Crassostrea gigas

Arnaud Tanguy ^{1, 2, *} Isabelle Boutet ² Pierre Boudry ³ Lionel Degremont ³ Jean Laroche ² Dario Moraga ²

* Auteur correspondant

1 ADMM - Adaptation et diversité en milieu marin

2 LEMAR - Laboratoire des Sciences de l'Environnement Marin (LEMAR)

3 LGP - Laboratoire de Génétique et Pathologie

Abstract : Phosphoglucomutase is a key enzyme in glycolysis and has been widely studied in vertebrates and some invertebrates but no molecular information is available in marine invertebrates despite the importance of this marker in ecological and genetical studies. In this work, we isolated a cDNA and the corresponding genomic sequence that encode PGM-2 locus in the Pacific oyster Crassostrea gigas. We used sequences drawn from the database to construct an evolutionary framework for examining the position of mollusc PGM sequences among prokaryotic and eukaryotic homologues and showed that oyster PGM gene organization was closer to vertebrates PGM genes than other invertebrates as previously found in other Lophotrochozoa species. We also investigated PGM mRNA expression in oyster tissues in response to xenobiotics (i.e hydrocarbons and pesticides). The results obtained showed that PGM mRNA expression is mostly up-regulated in the first steps of the response to pollutant exposure and is xenobiotic-dependant.

keyword : PGM PhosphoGlucoMutase UTR unTranslated Region ADI Atrazine-Diuron-Isoprouron

Type de document :

Article dans une revue

Gene, Elsevier, 2006, 382 (1 Novembre 2006), pp.20-27. 〈10.1016/j.gene.2006.06.005〉

Domaine :

Sciences de l'environnement

Liste complète des métadonnées

http://hal.univ-brest.fr/hal-00663786
Contributeur : Catherine Bertignac <>
Soumis le : vendredi 27 janvier 2012 - 15:18:39
Dernière modification le : vendredi 31 août 2018 - 09:14:09

Molecular identification and expression of the phosphoglucomutase (PGM) gene from the Pacific oyster Crassostrea gigas

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Molecular identification and expression of the phosphoglucomutase (PGM) gene from the Pacific oyster Crassostrea gigas

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