Nitrogen uptake and assimilation kinetics in Alexandrium minutum (Dynophyceae): effect of N-limited growth rate on nitrate and ammonium interactions

Jean-François Maguer 1, * Stéphane L'Helguen 1 Christian Madec 1 Claire Labry 2 Pierre Le Corre 1
* Auteur correspondant
1 Laboratoire de chimie marine
2 Dyneco - Département DYNamiques de l'Environnement CÔtier
Abstract : Uptake and assimilation kinetics of nitrate and ammonium were investigated along with inhibition of nitrate uptake by ammonium in the harmful dinoflagellate Alexandrium minutum Halim at different nitrogen (N)-limited growth rates. Alexandrium minutum had a strong affinity for nitrate and ammonium (Ks50.26 0.03 and 0.31 0.04 lmol .L 1, respectively) whatever the degree of N deficiency of the cells. Ammonium was always the preferred form of nitrogen taken up (rNO3=rNH4 50.42-0.50). In the presence of both forms, nitrate uptake was inhibited by ammonium, and inhibition was particularly marked in N-sufficient cells (Imax~0.9 and Ki50.31-0.56 lmol .L 1). In the case of N assimilation, ammonium was also the preferred form in N-deficient cells (rassim NO3=rassim NH4 50.54-0.72), whereas in N-sufficient cells, both N sources were equally preferred (rassim NO3=rassim NH4 50.90-1.00). The comparison of uptake and assimilation rates highlighted the ability of A. minutum to significantly store in 1 h nitrate and ammonium in amounts sufficient to supply twice the daily N requirements of the slowest-growing N-deficient cells. Nitrogen uptake kinetic parameters of A. minutum and their ecological implications are discussed.
Keywords : Alexandrium ammonium assimilation growth rate inhibition kinetics nitrate semicontinuous culture uptake
Type de document :
Article dans une revue
Journal of Phycology, Wiley, 2007, 43, pp.295-303. 〈10.1111/j.1529-8817.2007.00334.x〉
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Contributeur : Violaine Garguilo <>
Soumis le : mercredi 19 octobre 2011 - 10:05:55
Dernière modification le : mercredi 29 novembre 2017 - 15:11:40

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Jean-François Maguer, Stéphane L'Helguen, Christian Madec, Claire Labry, Pierre Le Corre. Nitrogen uptake and assimilation kinetics in Alexandrium minutum (Dynophyceae): effect of N-limited growth rate on nitrate and ammonium interactions. Journal of Phycology, Wiley, 2007, 43, pp.295-303. 〈10.1111/j.1529-8817.2007.00334.x〉. 〈hal-00633672〉

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