Comparative study of three analysis methods (TTGE, flow cytometry and HPLC) for xenobiotic impact assessment on phytoplankton communities

Sabine Stachowski-Haberkorn; Louis Quiniou; Beatriz Beker; Hansy Haberkorn; Dominique Marie; Denis De La Broise

doi:10.1007/s10646-008-0288-8

Comparative study of three analysis methods (TTGE, flow cytometry and HPLC) for xenobiotic impact assessment on phytoplankton communities

Sabine Stachowski-Haberkorn ^{1, 2, *} Louis Quiniou ^{1, 3} Beatriz Beker ⁴ Hansy Haberkorn ^{1, 3} Dominique Marie ⁵ Denis De La Broise ^{1, 3, *}

* Auteur correspondant

1 UEB - Université européenne de Bretagne

2 LUBEM - Laboratoire Universitaire de Biodiversité et Ecologie Microbienne

3 LEMAR - Laboratoire des Sciences de l'Environnement Marin (LEMAR)

4 COM - Centre d'océanologie de Marseille

5 ADMM - Adaptation et diversité en milieu marin

Abstract : The impacts of the fungicide Opus® (epoxiconazole) on marine phytoplankton communities were assessed in a 12-day field experiment using in situ microcosms maintained underwater at 6 m depth. Three community analysis methods were compared for their sensitivity threshold in fungicide impact detection. When phytoplankton communities were exposed to 1 μg l−1 of epoxiconazole, no effects could be demonstrated using TTGE (Temporal Temperature Gradient gel Electrophoresis), flow cytometry or HPLC. When exposed to 10 μg l−1, TTGE fingerprints from PCR amplified 18S rDNA of communities exhibited significant differences compared with controls (ANOSIM, P = 0.028). Neither flow cytometry counts, nor HPLC pigment profiles allowed to show significant differences in microcosms exposed to 10 μg l−1 of epoxiconazole. When exposed to 100 μg l−1, all three methods allowed to detect significant differences in treated microcosms, as compared to controls. The TTGE analysis appears in this study as the most sensitive method for fungicide impact assessment on eukaryote microbial communities.

Keywords : Microcosm Phytoplankton community Epoxiconazole TTGE Flow cytometry HPLC

Type de document :

Article dans une revue

Ecotoxicology, Springer Verlag, 2009, 18 (3), pp.364-376. 〈10.1007/s10646-008-0288-8〉

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http://hal.univ-brest.fr/hal-00451105
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Comparative study of three analysis methods (TTGE, flow cytometry and HPLC) for xenobiotic impact assessment on phytoplankton communities

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Comparative study of three analysis methods (TTGE, flow cytometry and HPLC) for xenobiotic impact assessment on phytoplankton communities

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