Electrochemistry of cytochrome c immobilized on cardiolipin-modified electrodes: a probe for protein – lipid interactions

Abstract : Electrochemistry of cytochrome c (cyt c) immobilized on a cardiolipin (CL) / phosphatidylcholine (PC) film supported on a glassy carbon electrode was investigated using variable-frequency AC voltammetry. At low ionic strength, we observed two redox-active subpopulations characterized by distinct values of potential (E1/2) and electron transfer rate constant (kET). At high ionic strength, only one subpopulation was detected, consistent with the existence of very stable cyt c – CL adducts, most probably formed by hydrophobic interactions between the protein and the fatty acid (FA) chains carried by CL. This subpopulation exhibits a comparatively high kET value (> 300 s−1) apparently changing with the structure of the FA chains of CL, i.e. 18:2(n-6) or 14:0. Our study suggests that electrochemistry can be a useful technique for probing protein − lipid interactions, and more particularly the role played by the specific structure of the FA chains of CL on cyt c binding.
Type de document :
Pré-publication, Document de travail
2015
Liste complète des métadonnées

Littérature citée [38 références]  Voir  Masquer  Télécharger

http://hal.univ-brest.fr/hal-01130700
Contributeur : Frederic Gloaguen <>
Soumis le : jeudi 12 mars 2015 - 11:18:38
Dernière modification le : jeudi 11 janvier 2018 - 06:22:24
Document(s) archivé(s) le : lundi 17 avril 2017 - 11:06:25

Fichier

Gloaguen_BBA_2013.pdf
Fichiers produits par l'(les) auteur(s)

Identifiants

Citation

Antoine Perhirin, Edouard Kraffe, Yanic Marty, Francois Quentel, Philippe Elies, et al.. Electrochemistry of cytochrome c immobilized on cardiolipin-modified electrodes: a probe for protein – lipid interactions. 2015. 〈hal-01130700〉

Partager

Métriques

Consultations de la notice

433

Téléchargements de fichiers

189