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Journal Articles Limnology and Oceanography: Methods Year : 2024

Comparison of methods for DMSP measurements in dinoflagellate cultures

Abstract

A comparison of three analytical methods (the indirect GC‐FPD and MIMS, and direct LC‐MS/MS) for dimethylsulfoniopropionate (DMSP) measurements was conducted to assess their accuracy and reliability. The three methods showed a linear response but are distinguished by their linearity range, the largest being for MIMS. All three methods showed good precision on Alexandrium minutum samples (2–12%). The variability between the three methods when comparing analyses of A. minutum replicates was 11%, with the DMSP measurements by LC‐MS/MS being the highest. This result also confirms that indirect DMSP measurement after hydrolysis for GC or MIMS methods does not lead to an overestimation of DMSP values in A. minutum . A special focus was made on the more recent LC‐MS/MS method including further assays in sample preparation and storage from cultures of the dinoflagellate A. minutum . Dinoflagellate cells should be harvested by gentle filtration (< 5 cm Hg) or slow centrifugation (500 × g ) to retrieve the largest DMSP pool. For the LC‐MS/MS method, MeOH used for cell extraction should be added prior to freezing (to prevent DMSP degradation). Samples will then be stable in frozen storage for at least 2 months. Finally, direct and indirect methods are complementary for identifying the exact DMSP fraction among dimethylsulfide‐producing compounds that compose total and particulate DMSP pools issued from newly screened organisms or environmental samples.
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hal-04567173 , version 1 (06-05-2024)

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Amandine Caruana, Eva Bucciarelli, Céline Deleporte, Emilie Le Floc'H, Fabienne Hervé, et al.. Comparison of methods for DMSP measurements in dinoflagellate cultures. Limnology and Oceanography: Methods, 2024, ⟨10.1002/lom3.10618⟩. ⟨hal-04567173⟩
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