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Journal Articles Frontiers in Nuclear Medicine Year : 2021

Fully Automated 68Ga-Labeling and Purification of Macroaggregated Albumin Particles for Lung Perfusion PET Imaging

Frédérique Blanc-Béguin
  • Function : Author
Julien Masset
  • Function : Author
Philippe Robin
  • Function : Author
Simon Hennebicq
  • Function : Author
Valérie Guilloux
  • Function : Author
Charles Vriamont
  • Function : Author
Corentin Warnier
  • Function : Author
Virginie Cogulet
  • Function : Author
Peter Eu
  • Function : Author
Pierre-Yves Salaün
  • Function : Author
Pierre-Yves Le Roux
  • Function : Author

Abstract

Lung PET/CT is a promising imaging modality for regional lung function assessment. Our aim was to develop and validate a fast, simple, and fully automated GMP compliant [ 68 Ga]Ga-MAA labeling procedure, using a commercially available [ 99m Tc]Tc-MAA kit, a direct gallium-68 eluate and including a purification of the [ 68 Ga]Ga-MAA. Method: The synthesis parameters (pH, heating temperature) were manually determined. Automated 68 Ga-labeling of MAA was then developed on a miniAIO (Trasis®, Ans, Belgium) module. An innovative automated process was developed for the purification. The process was then optimized and adapted to automate both the [ 68 Ga]Ga-MAA synthesis and the isolation of gallium-68 eluate required for the pulmonary ventilation PET/CT. Results: The 15-min process demonstrated high reliability and reproducibility, with high synthesis yield (>95 %). Mean [ 68 Ga]Ga-MAA radiochemical purity was 99 % ± 0.6 %. The 68 Ga-labeled MAA particles size and morphology remained unchanged. Conclusion: A fast, user friendly, and fully automated process to produce GMP [ 68 Ga]Ga-MAA for clinical use was developed. This automated process combining the advantages of using a non-modified MAA commercial kit, a gallium-68 eluate without pre-purification and an efficient final purification of the [ 68 Ga]Ga-MAA may facilitate the implementation of lung PET/CT imaging in nuclear medicine departments.

Dates and versions

hal-04545867 , version 1 (14-04-2024)

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Cite

Frédérique Blanc-Béguin, Julien Masset, Philippe Robin, Raphaël Tripier, Simon Hennebicq, et al.. Fully Automated 68Ga-Labeling and Purification of Macroaggregated Albumin Particles for Lung Perfusion PET Imaging. Frontiers in Nuclear Medicine, 2021, 1 (6), pp.409-411. ⟨10.3389/fnume.2021.778191⟩. ⟨hal-04545867⟩
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