Effects of oleic acid on the high threshold barium current in seabass Dicentrarchus labrax ventricular myocytes - Université de Bretagne Occidentale Access content directly
Journal Articles Journal of Experimental Biology Year : 2006

Effects of oleic acid on the high threshold barium current in seabass Dicentrarchus labrax ventricular myocytes

Abstract

SUMMARY The present study employed a patch clamp technique in isolated seabass ventricular myocytes to investigate the hypothesis that oleic acid (OA), a mono-unsaturated fatty acid, can exert direct effects upon whole-cell barium currents. Acute application of free OA caused a dose-dependent depression of the whole-cell barium current that was evoked by a voltage step to 0 mV from a holding potential of –80 mV. The derived 50% inhibitory concentration(IC50) was 12.49±0.27 μmol l–1. At a concentration of 30 μmol l–1, OA significantly reduced the current density to about 45% of control values, but did not modify either the shape of the current-density voltage relationship or the apparent reversal potential. In addition, OA did not modify the voltage dependence of either steady state inactivation or activation curves. Taken together, these results indicate that physiological concentrations of free OA decrease the conductance of the L-type inward current, without altering its properties of selectivity and its voltage dependence. The inhibitory effect of OA upon the L-type calcium channel may translate, in vivo, into a protective effect against arrhythmias induced by Ca2+ overload.

Dates and versions

hal-04477969 , version 1 (26-02-2024)

Identifiers

Cite

A. Chatelier, N. Imbert, Jose-Luis Zambonino-Infante, D. Mckenzie, P. Bois. Effects of oleic acid on the high threshold barium current in seabass Dicentrarchus labrax ventricular myocytes. Journal of Experimental Biology, 2006, 209 (20), pp.4033-4039. ⟨10.1242/jeb.02470⟩. ⟨hal-04477969⟩
6 View
0 Download

Altmetric

Share

Gmail Facebook X LinkedIn More