Scale-Up to Pilot of a Non-Axenic Culture of Thraustochytrids Using Digestate from Methanization as Nitrogen Source - Université de Bretagne Occidentale Access content directly
Journal Articles Marine drugs Year : 2022

Scale-Up to Pilot of a Non-Axenic Culture of Thraustochytrids Using Digestate from Methanization as Nitrogen Source

Thibaud Vinet
  • Function : Author

Abstract

The production of non-fish based docosahexaenoic acid (DHA) for feed and food has become a critical need in our global context of over-fishing. The industrial-scale production of DHA–rich Thraustochytrids could be an alternative, if costs turned out to be competitive. In order to reduce production costs, this study addresses the feasibility of the non-axenic (non-sterile) cultivation of Aurantiochytrium mangrovei on industrial substrates (as nitrogen and mineral sources and glucose syrup as carbon and energy sources), and its scale-up from laboratory (250 mL) to 500 L cultures. Pilot-scale reactors were airlift cylinders. Batch and fed-batch cultures were tested. Cultures over 38 to 62 h achieved a dry cell weight productivity of 3.3 to 5.5 g.L−1.day−1, and a substrate to biomass yield of up to 0.3. DHA productivity ranged from 10 to 0.18 mg.L−1.day−1. Biomass productivity appears linearly related to oxygen transfer rate. Bacterial contamination of cultures was low enough to avoid impacts on fatty acid composition of the biomass. A specific work on microbial risks assessment (in supplementary files) showed that the biomass can be securely used as feed. However, to date, there is a law void in EU legislation regarding the recycling of nitrogen from digestate from animal waste for microalgae biomass and its usage in animal feed. Overall, the proposed process appears similar to the industrial yeast production process (non-axenic heterotrophic process, dissolved oxygen supply limiting growth, similar cell size). Such similarity could help in further industrial developments.
Fichier principal
Vignette du fichier
marinedrugs-20-00499.pdf (4.81 Mo) Télécharger le fichier
Origin : Publisher files allowed on an open archive

Dates and versions

hal-04237349 , version 1 (12-10-2023)

Licence

Attribution

Identifiers

Cite

Denis de La Broise, Mariana Ventura, Luc Chauchat, Maurean Guerreiro, Teo Michez, et al.. Scale-Up to Pilot of a Non-Axenic Culture of Thraustochytrids Using Digestate from Methanization as Nitrogen Source. Marine drugs, 2022, 20 (8), pp.499. ⟨10.3390/md20080499⟩. ⟨hal-04237349⟩
39 View
10 Download

Altmetric

Share

Gmail Facebook X LinkedIn More