Alternative to High Pressure Mercury Vapor Lamp for Photo Induced Fluorescence Analytical Methods; Application to the Determination of Pesticides in Water
Abstract
The photo-induced fluorescence method is often applied to determine non-native fluorescent compounds. It typically uses
UV irradiation from a high pressure mercury vapor discharge lamp to create photo-induced fluorescent compounds, which
are then quantified by fluorescence spectroscopy. However, these mercury lamps require a high-voltage power supply and
may accidentally induce electric shocks and the release of mercury vapors. As an alternative, we have evaluated in this
technical note new UV-C germicidal lamps. These lamps exhibit a higher power at 254 nm and allowed us to obtain a far
greater amount of photo-induced compounds in a shorter time. For the first time, this new irradiation system has been
applied for the determination of pesticides in water and has shown a significant increase in the method sensitivity. These
good results allowed us to conclude that the new UV-C lamps are a relevant alternative to high pressure mercury vapor
discharge lamps for use with photo induced fluorescent m
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