Highly stable and inert complexation of Indium(III) by reinforced cyclam dipicolinate and a bifunctional derivative for bead encoding in mass cytometry

Abstract : H2cb‐te2pa, a cross‐bridged cyclam functionalized by two picolinate arms, was used for the formation of an incredible inert In(III) chelate. For the first time a bifunctional analogue of H2cb‐te2pa was prepared by C‐functionalization to keep its coordination properties intact. However, this strategy leads to the formation of two diastereoisomers, as evidenced and studied by NMR experiments and DFT calculations. Kinetic studies proved nevertheless that both isomers of the complex are equally inert; they were therefore used without distinction for their covalent grafting on polystyrene beads. The so‐called metal‐encoded beads were tested for Imaging Mass Cytometry. The detection of 115In allows the generation of images with high quality, proving the great potential of the bifunctional [In(cb‐te2pa)]+ derivatives for single‐cell analysis by mass cytometry.
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https://hal.univ-brest.fr/hal-02281241
Contributor : Nicolas Renard <>
Submitted on : Monday, September 9, 2019 - 8:00:29 AM
Last modification on : Wednesday, October 30, 2019 - 3:54:02 PM

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Laura Grenier, Maryline Beyler, Carlos Platas-Iglesias, Taunia Closson, David Esteban Gómez, et al.. Highly stable and inert complexation of Indium(III) by reinforced cyclam dipicolinate and a bifunctional derivative for bead encoding in mass cytometry. Chemistry - A European Journal, Wiley-VCH Verlag, 2019, ⟨10.1002/chem.201903969⟩. ⟨hal-02281241⟩

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