Combining a pyclen framework with conjugated antenna for the design of europium and samarium luminescent bioprobes
Abstract
The first pyclen based ligand bearing two picolinate intra-ligand charge transfer transition antennae and one acetate arm organized in a dissymmetric manner was synthesized for Eu(III) and Sm(III) complexation. The europium complex presents an excellent brightness and biphotonic imaging of T24-cells has been performed using Eu(III) and the less common Sm(III) bioprobes.
Un premier ligand à base de pyclen portant deux bras antennes picolinate à transfert de charge intra-ligand et un bras acétate organisé de manière dissymétrique a été synthétisé pour la complexation de l’Eu (III) et du Sm (III) pour former des sondes biologiques de lanthanides mono- et bi-photoniques utilisés dans l'imagerie 2P de cellules T24 (PFA) -fixées.