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Journal articles
Influence of zinc on soluble carbohydrate and free amino acid levels in rapeseed plants regenerated in vitro in the presence of zinc
Abstract : To gain more insight into the impact of zinc on the primary metabolites in rapeseed, plants were regenerated in vitro in the presence of zinc (0.1–1 mM), acclimatized, transferred to a greenhouse, and treated with 2 mM ZnSO4. The main metabolites, including soluble carbohydrates and free amino acids, were analyzed by nuclear magnetic resonance spectroscopy, and further confirmed by spectrophotometry and enzymatic analyses. Exposure of these greenhouse-grown plants to ZnSO4 led to both a significant rise of the total amino acid level and an increase of proline accumulation in the different tissues. Moreover, the control plants, regenerated without zinc stress, exhibited a significant reduction of soluble carbohydrate levels, whereas the plants derived from Zn-treated regenerants exhibited significantly higher levels of both glucose and sucrose. These levels increased proportionally with the Zn concentration in the regeneration step.
https://hal.univ-brest.fr/hal-01577358
Contributor : Nicolas Renard <>
Submitted on : Friday, August 25, 2017 - 3:12:39 PM
Last modification on : Thursday, November 29, 2018 - 4:09:16 PM
Contributor : Nicolas Renard <>
Submitted on : Friday, August 25, 2017 - 3:12:39 PM
Last modification on : Thursday, November 29, 2018 - 4:09:16 PM
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