Euphotic layer dinitrogen (N-2) fixation and primary production (PP) were measured in the eastern Atlantic Ocean (38 degrees N-21 degrees S) using N-15(2) and C-13 bicarbonate tracer incubations. This region is influenced by Saharan dust deposition and waters with low nitrogen to phosphorus (N/P) ratios originating from the Subantarctic and the Benguela upwelling system. Depth-integrated rates of N-2 fixation in the north (0 degrees N-38 degrees N) ranged from 59 to 370 mu mol N m(-2) d(-1), with the maximal value at 19 degrees N under the influence of the northwest African upwelling. Diazotrophic activity in the south (0 degrees S-21 degrees S), though slightly lower, was surprisingly close to observations in the north, with values ranging from 47 to 119 mu mol N m(-2) d(-1). Our North Atlantic N-2 fixation rates correlate well with dust deposition, while those in the South Atlantic correlate strongly with excess phosphate relative to nitrate. There, the necessary iron is assumed to be supplied from the Benguela upwelling system. When converting N-2 fixation to carbon uptake using a Redfield ratio (6.6), we find that N-2 fixation may support up to 9% of PP in the subtropical North Atlantic (20 degrees N-38 degrees N), 5% in the tropical North Atlantic (0 degrees N-20 degrees N), and 1% of PP in the South Atlantic (0 degrees S-21 degrees S). Combining our data with published data sets, we estimate an annual N input of 27.610 Tg N yr(-1) over the open Atlantic Ocean, 11% of which enters the region between 20 degrees N and 50 degrees N, 71% between 20 degrees N and 10 degrees S, and 18% between 10 degrees N and 45 degrees S.