Flow cytometric assessment of morphology, viability, and production of reactive oxygen species of Crassostrea gigas oocytes. Application to Toxic dinoflagellate (Alexandrium minutum) exposure.

Nelly Le Goïc 1 Hélène Hégaret 1 Myrina Boulais 2 Jean-Philippe Béguel 1 Christophe Lambert 1 Caroline Fabioux 1 Philippe Soudant 1
1 LEMAR - Laboratoire des Sciences de l'Environnement Marin (LEMAR)
2 LPI - Laboratoire de Physiologie des Invertébrés
Abstract : The Pacific oyster Crassostrea gigas accounts for a large part of shellfish aquaculture production worldwide. Aspects of morphological and functional characteristics of oyster oocytes remain poorly documented, and traditional techniques, such as microscopic observations of shape or fertilization rate, are time and space consuming. The purpose of this study was to assess for the first time viability and reactive oxygen species (ROS) production of Pacific oyster oocytes using flow cytometry (FCM) and to apply this method to determine oocyte responses to in vitro exposure to the toxic dinoflagellate Alexandrium minutum. A culture of A. minutum caused a significant increase in oocyte ROS production, which gradually increased with the age of the culture, but viability was not affected. Effect of the supernatant of the same A. minutum culture did not cause any significant modifications of oocyte morphology, viability, or ROS level. This study confirmed that some oocyte cellular characteristics can be assessed using FCM techniques.
Keywords : ROS production Pacific oyster gamete quality viability flow cytometry ecotoxicology
Type de document :
Article dans une revue
Cytometry Part A, Wiley, 2014, 85A (12), pp.1049-56
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http://hal.univ-brest.fr/hal-01096877
Contributeur : Adminhal Univbrestbu <>
Soumis le : jeudi 18 décembre 2014 - 13:33:08
Dernière modification le : jeudi 11 janvier 2018 - 06:22:24

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  • HAL Id : hal-01096877, version 1
  • PUBMED : 25327519

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Nelly Le Goïc, Hélène Hégaret, Myrina Boulais, Jean-Philippe Béguel, Christophe Lambert, et al.. Flow cytometric assessment of morphology, viability, and production of reactive oxygen species of Crassostrea gigas oocytes. Application to Toxic dinoflagellate (Alexandrium minutum) exposure.. Cytometry Part A, Wiley, 2014, 85A (12), pp.1049-56. 〈hal-01096877〉

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