Rapid geographical differentiation of the European spread brown macroalga Sargassum muticum using HRMAS NMR and Fourier-Transform Infrared spectroscopy

Anaëlle Tanniou 1 Laurent Vandanjon 2, 3 Olivier Gonçalves 3 Nelly Kervarec 4 Valérie Stiger-Pouvreau 1, *
* Auteur correspondant
1 LEMAR - Laboratoire des Sciences de l'Environnement Marin (LEMAR)
2 LBCM - Laboratoire de Biotechnologie et Chimie Marines
3 GEPEA - Laboratoire de génie des procédés - environnement - agroalimentaire
4 Service de Résonance Magnétique Nucléaire
Abstract : Two recent techniques based on chemical footprinting analysis, HRMAS NMR and FTIR spectroscopy, were tested on a brown macroalgal model. These powerful and easily-to-use techniques allowed us to discriminate Sargassum muticum specimens collected in five different countries along Atlantic coasts, from Portugal to Norway. HRMAS NMR and FTIR permitted the obtaining of an overview of metabolites produced by the alga. Based on spectra analysis, results allowed us to successfully group the samples according to their geographical origin. HRMAS NMR and FTIR spectroscopy respectively point out the relation between the geographical localization and the chemical composition and demonstrated macromolecules variations regarding to environmental stress. Then, our results are discussed in regard of the powerful of these techniques together with the variability of the main molecules produced by Sargassum muticum along the Atlantic coasts.
Keywords : NMR Sargassum muticum FTIR spectroscopy Geographical origin discrimination HRMAS Latitudinal gradient
Type de document :
Article dans une revue
Talanta, Elsevier, 2015, 132, pp.451-456
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http://hal.univ-brest.fr/hal-01089290
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Soumis le : lundi 1 décembre 2014 - 14:47:10
Dernière modification le : lundi 16 avril 2018 - 15:22:02

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Anaëlle Tanniou, Laurent Vandanjon, Olivier Gonçalves, Nelly Kervarec, Valérie Stiger-Pouvreau. Rapid geographical differentiation of the European spread brown macroalga Sargassum muticum using HRMAS NMR and Fourier-Transform Infrared spectroscopy. Talanta, Elsevier, 2015, 132, pp.451-456. 〈hal-01089290〉

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