Immune responses of phenoloxidase and superoxide dismutase in the manila clam Venerupis philippinarum challenged with Vibrio tapetis - Part I: Spatio-temporal evolution of enzymes' activities post-infection

Cédric Le Bris 1, * Gaëlle Richard 1 Christine Paillard 1 Christophe Lambert 1 Catherine Seguineau 2 Olivier Gauthier 1 Fabrice Pernet 2 Fabienne Guérard 1
* Auteur correspondant
1 LEMAR - Laboratoire des Sciences de l'Environnement Marin (LEMAR)
2 LPI - Laboratoire de Physiologie des Invertébrés
Abstract : Manila clams, Venerupis philippinarum (Adams and Reeve, 1850), were experimentally challenged with two Vibrio tapetis strains: CECT4600T, the causative agent of Brown Ring Disease (BRD); and LP2 supposedly non-pathogenic in V.philippinarum. Changes in phenoloxidase (PO) and superoxide dismutase (SOD), two major enzymes involved in immunity, were studied in two tissues, the mantle and hemolymph for 30 days after infection in the extrapallial cavity. Bacterial infection in V.philippinarum resulted in modulation of PO and SOD activities that was both tissue- and time-dependent. A response at early times was detected in the mantle and was associated with significant increases in PO and SOD activities in LP2- and CECT4600T-challenged clams 36 h post injection. This first response in the mantle could be explained by the proximity to the injection region (extrapallial cavity). In the hemolymph the response occurred at later times and was associated with an increase in PO activity and a decrease in SOD activity. As hemolymph is a circulating fluid, this response delay could be due to an "integration time" needed by the organism to counteract the infection. Injections also impacted PO and SOD activities in both tissues and confirmed a difference in pathogenicity between the two V.tapetis strains.
Keywords : Phenoloxidase Superoxide dismutase Venerupis philippinarum Vibrio tapetis Immunity
Type de document :
Article dans une revue
Fish and Shellfish Immunology, Elsevier, 2015, 42 (1), pp.16-24
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http://hal.univ-brest.fr/hal-01089239
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Soumis le : lundi 1 décembre 2014 - 14:09:08
Dernière modification le : jeudi 11 janvier 2018 - 06:22:24

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Cédric Le Bris, Gaëlle Richard, Christine Paillard, Christophe Lambert, Catherine Seguineau, et al.. Immune responses of phenoloxidase and superoxide dismutase in the manila clam Venerupis philippinarum challenged with Vibrio tapetis - Part I: Spatio-temporal evolution of enzymes' activities post-infection. Fish and Shellfish Immunology, Elsevier, 2015, 42 (1), pp.16-24. 〈hal-01089239〉

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