New genetic markers to identify European resistant abalone to vibriosis revealed by high-resolution melting analysis, a sensitive and fast approach

Anne-Leila Meistertzheim 1, 2 Isabelle Calves 2 Véronique Roussel 3 Alain Van Wormhoudt 3 Jean Laroche 2 Sylvain Huchette 4 Christine Paillard 2
1 CEFREM - Centre de formation et de recherche sur l'environnement marin
2 LEMAR - Laboratoire des Sciences de l'Environnement Marin (LEMAR)
3 Station de Biologie Marine de Concarneau
4 France Haliotis
Abstract : Increasing temperature of seawater is often associated with increased exposure incidence of disease in field and in aquaculture populations. Numerous episodic mass mortalities of the abalone Haliotis tuberculata have been observed along the northern Brittany coast of France caused by a complex interaction between the host, pathogen and environmental factors. Here, we evaluated the potential of high-resolution melting (HRM) analysis for mutation genotyping and development of genetic markers for resistance to vibriosis in the gastropod species H. tuberculata. Small amplicon assays were developed and revealed genetic polymorphism between surviving and susceptible abalone obtained after two successive infections of aquaculture families in controlled conditions. Together with specific COI haplotypes, we identified particular genotypes in nascent polypeptide-associated complex subunit alpha and ferritin genes linked to the susceptibility or resistance of abalone to vibriosis. Selection of genitors based on these genes may increase the proportion in offspring of resistant individuals of more than 76 %. Finally, HRM assays constitute a very efficient genotyping tool to validate the genetic markers on a representative number of individuals of wild populations and thus identify future resistant genitors for aquaculture or conservation purposes
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Article dans une revue
Marine Biology, Springer Verlag, 2014, 8, pp.1883-1893. 〈10.1007/s00227-014-2470-2〉
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Soumis le : mardi 8 juillet 2014 - 17:16:14
Dernière modification le : mardi 29 mai 2018 - 12:50:24

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Anne-Leila Meistertzheim, Isabelle Calves, Véronique Roussel, Alain Van Wormhoudt, Jean Laroche, et al.. New genetic markers to identify European resistant abalone to vibriosis revealed by high-resolution melting analysis, a sensitive and fast approach. Marine Biology, Springer Verlag, 2014, 8, pp.1883-1893. 〈10.1007/s00227-014-2470-2〉. 〈hal-01020946〉

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