Skip to Main content Skip to Navigation
Journal articles

Diagnostic accuracy of blood B-cell subset profiling and autoimmunity markers in Sjögren's syndrome.

Abstract : The aims of this study were to evaluate the diagnostic accuracy of blood B-cell subset profiling and immune-system activation marker assays in primary Sjögren's syndrome (pSS) and to assess whether adding these tools to the current laboratory item would improve the American-European Consensus Group (AECG) criteria. METHODS: In a single-center cohort of patients with suspected pSS, we tested the diagnostic performance of anti-SSA, antinuclear antibody (ANA), rheumatoid factor (RF), gammaglobulins, IgG titers, and B-cell ratio defined as (Bm2 + Bm2')/(eBm5 + Bm5), determined using flow cytometry. The reference standard was a clinical diagnosis of pSS established by a panel of experts. RESULTS: Of 181 patients included in the study, 77 had pSS. By logistic regression analysis, only ANA ≥1:640 (sensitivity, 70.4%; specificity 83.2%) and B-cell ratio ≥5 (sensitivity, 52.1%; specificity, 83.2%) showed independent associations with pSS of similar strength. In anti-SSA-negative patients, presence of either of these two criteria had 71.0% sensitivity but only 67.3% specificity for pSS; whereas combining both criteria had 96.2% specificity but only 12.9% sensitivity. Adding either of these two criteria to the AECG criteria set increased sensitivity from 83.1% to 90.9% but decreased specificity from 97.1% to 85.6%, whereas adding both criteria in combination did not substantially modify the diagnostic performance of the criteria set. The adjunction of RF + ANA ≥1:320, as proposed in the new American College of Rheumatology (ACR) criteria, did not improve the diagnostic value of anti-SSA. CONCLUSIONS: Blood B-cell subset profiling is a simple test that has good diagnostic properties for pSS. However, adding this test, with or without ANA positivity, does not improve current classification criteria.
Document type :
Journal articles
Complete list of metadatas

Cited literature [18 references]  Display  Hide  Download

https://hal.univ-brest.fr/hal-00987614
Contributor : Geneviève Michel <>
Submitted on : Thursday, February 4, 2016 - 8:39:11 AM
Last modification on : Wednesday, July 18, 2018 - 11:09:55 AM
Long-term archiving on: : Saturday, November 12, 2016 - 7:39:36 AM

File

cornec_et_al_ART_2014.pdf
Publisher files allowed on an open archive

Identifiers

Collections

Citation

Divi Cornec, Alain Saraux, Jacques-Olivier Pers, Sandrine Jousse-Joulin, Thierry Marhadour, et al.. Diagnostic accuracy of blood B-cell subset profiling and autoimmunity markers in Sjögren's syndrome.. Arthritis Research and Therapy, BioMed Central, 2014, 16 (1), pp.R15. ⟨10.1186/ar4442⟩. ⟨hal-00987614⟩

Share

Metrics

Record views

242

Files downloads

438