Effects of sangre de drago in an in vitro model of cutaneous neurogenic inflammation.

Ulysse Pereira 1 Caridad Garcia-Le Gal 2 Grégoire Le Gal 3, 4 Nicholas Boulais 1 Nicolas Lebonvallet 1 Germaine Dorange 1 Luc Lefeuvre 5 Agnés Gougerot 5 Laurent Misery 2
1 Facteurs nerveux et structuration tissulaire
2 Service de dermatologie
3 GETBO - Groupe d'Etude de la Thrombose de Bretagne Occidentale
4 DMIP - Brest - Département de Médecine Interne et Pneumologie [Brest]
5 URIAGE - Dermato - Dermatological Laboratories of Uriage
Abstract : Sangre de drago (SD) is a viscous bright red resin collected from Croton lechleri trees that grow in the South American jungle. This sap is used extensively in the native pharmacopoeia to treat skin disorders. Its effectiveness as an inhibitor of neurogenic inflammation has been recently demonstrated. To understand the underlying mechanisms of these effects, we examined the ability of SD to reduce substance P (SP) release in an in vitro model of cutaneous neurogenic inflammation (CNI). This model is based on an enzyme immunoassay of SP (an inducer of CNI) in a porcine co-culture of dorsal root ganglion neurons and keratinocytes. After incubation with different concentrations of SD, we noted an immediate and significant dose-dependent decrease in basal SP release, with average values of 32% at 1% SD (v/v) and 26% at 0.1% (v/v). On the other hand, pretreatment (72 or 1 h) of the co-culture with 1% SD (v/v) was sufficient to induce a 111% (72 h) or 65% (1 h) inhibition of capsaicin-induced SP release, while 0.1% SD (v/v) triggered a 109% (72 h) or 30% (1 h) inhibition. We conclude that sangre de drago is a potent inhibitor of CNI through direct inhibition of neuropeptide release by sensory afferent nerves.
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Experimental Dermatology, Wiley, 2010, 19 (9), pp.796-9. 〈10.1111/j.1600-0625.2010.01090.x〉
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Contributeur : Ghislaine Calvez <>
Soumis le : mardi 14 janvier 2014 - 13:37:05
Dernière modification le : mercredi 10 janvier 2018 - 14:42:02

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Ulysse Pereira, Caridad Garcia-Le Gal, Grégoire Le Gal, Nicholas Boulais, Nicolas Lebonvallet, et al.. Effects of sangre de drago in an in vitro model of cutaneous neurogenic inflammation.. Experimental Dermatology, Wiley, 2010, 19 (9), pp.796-9. 〈10.1111/j.1600-0625.2010.01090.x〉. 〈hal-00930151〉

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