Evaluation of neovessels in atherosclerotic plaques of rabbits using an albumin-binding intravascular contrast agent and MRI. - Université de Bretagne Occidentale Access content directly
Journal Articles Journal of Magnetic Resonance Imaging Year : 2008

Evaluation of neovessels in atherosclerotic plaques of rabbits using an albumin-binding intravascular contrast agent and MRI.

Claudia Calcagno
  • Function : Author
Karen C Briley-Saebo
  • Function : Author
James Tunstead
  • Function : Author
Juan-Gilberto S Aguinaldo
  • Function : Author
Venkatesh Mani
  • Function : Author
Vito Lorusso
  • Function : Author
Friedrich M Cavagna
  • Function : Author
Zahi A Fayad
  • Function : Author

Abstract

PURPOSE: To test whether B-22956/1, a novel intravascular contrast agent with a high affinity to serum albumin (Bracco Imaging SpA.), allowed quantifying neovessel and macrophage density in atherosclerotic plaques of rabbits using MRI. MATERIALS AND METHODS: A T1-weighted MRI of the aorta was acquired in 10 rabbits (7 atherosclerotic and 3 control rabbits) before and up to 2 h after intravenous injection of 100 mumol/kg of Gd-DTPA or 75 mumol/kg of B-22956/1. Plaque enhancement was measured at different time points. Immunohistochemistry was performed using anti-CD 31 antibodies and anti-RAM 11 antibodies to correlate to neovessel and macrophage density, respectively. RESULTS: MRI showed a significant plaque enhancement 2 h after B-22956/1 versus Gd-DTPA in the atherosclerotic group (39.75% versus 9.5%; P < 0.0001. Early atherosclerotic plaques (n = 146) enhancement positively correlates with neovessel density on corresponding histological sections (r = 0.42; P < 0.01). Enhancement of atherosclerotic plaques 2 h after injection of B-22956/1 correlated with macrophage density (r = 0.71; P < 0.01). CONCLUSION: Enhancement of atherosclerotic plaques with MRI correlated with neovessel density at early time points after the injection of B-22956/1 and with macrophage density, at later time points. Hence, B-22956/1-enhanced MRI represents a promising imaging technique for the identification of "high-risk" plaques.

Dates and versions

hal-00837632 , version 1 (23-06-2013)

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Jean-Christophe Cornily, Fabien Hyafil, Claudia Calcagno, Karen C Briley-Saebo, James Tunstead, et al.. Evaluation of neovessels in atherosclerotic plaques of rabbits using an albumin-binding intravascular contrast agent and MRI.. Journal of Magnetic Resonance Imaging, 2008, 27 (6), pp.1406-11. ⟨10.1002/jmri.21369⟩. ⟨hal-00837632⟩
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