Evaluation of neovessels in atherosclerotic plaques of rabbits using an albumin-binding intravascular contrast agent and MRI.

Jean-Christophe Cornily 1, 2 Fabien Hyafil 3 Claudia Calcagno Karen C Briley-Saebo James Tunstead Juan-Gilberto S Aguinaldo Venkatesh Mani Vito Lorusso Friedrich M Cavagna Zahi A Fayad
1 CHRU - Cardio - Département de Cardiologie
2 ORPHY (EA 4324) - Optimisation des régulations physiologiques
3 Service de cardiologie
Abstract : PURPOSE: To test whether B-22956/1, a novel intravascular contrast agent with a high affinity to serum albumin (Bracco Imaging SpA.), allowed quantifying neovessel and macrophage density in atherosclerotic plaques of rabbits using MRI. MATERIALS AND METHODS: A T1-weighted MRI of the aorta was acquired in 10 rabbits (7 atherosclerotic and 3 control rabbits) before and up to 2 h after intravenous injection of 100 mumol/kg of Gd-DTPA or 75 mumol/kg of B-22956/1. Plaque enhancement was measured at different time points. Immunohistochemistry was performed using anti-CD 31 antibodies and anti-RAM 11 antibodies to correlate to neovessel and macrophage density, respectively. RESULTS: MRI showed a significant plaque enhancement 2 h after B-22956/1 versus Gd-DTPA in the atherosclerotic group (39.75% versus 9.5%; P < 0.0001. Early atherosclerotic plaques (n = 146) enhancement positively correlates with neovessel density on corresponding histological sections (r = 0.42; P < 0.01). Enhancement of atherosclerotic plaques 2 h after injection of B-22956/1 correlated with macrophage density (r = 0.71; P < 0.01). CONCLUSION: Enhancement of atherosclerotic plaques with MRI correlated with neovessel density at early time points after the injection of B-22956/1 and with macrophage density, at later time points. Hence, B-22956/1-enhanced MRI represents a promising imaging technique for the identification of "high-risk" plaques.
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Journal of Magnetic Resonance Imaging, Wiley-Blackwell, 2008, 27 (6), pp.1406-11. 〈10.1002/jmri.21369〉
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Contributeur : François Guerrero <>
Soumis le : dimanche 23 juin 2013 - 18:42:52
Dernière modification le : jeudi 13 septembre 2018 - 15:17:13

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UNIV-BREST | USPC | ORPHY

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Jean-Christophe Cornily, Fabien Hyafil, Claudia Calcagno, Karen C Briley-Saebo, James Tunstead, et al.. Evaluation of neovessels in atherosclerotic plaques of rabbits using an albumin-binding intravascular contrast agent and MRI.. Journal of Magnetic Resonance Imaging, Wiley-Blackwell, 2008, 27 (6), pp.1406-11. 〈10.1002/jmri.21369〉. 〈hal-00837632〉

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