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In Sjögren's syndrome, B lymphocytes induce epithelial cells of salivary glands into apoptosis through protein kinase C delta activation.

Abstract : Sjögren's syndrome (SS) is a chronic autoimmune epithelitis associated with diffuse lymphocytic infiltration that varies in composition and differs according to lesion severity. T lymphocytes have been viewed as competent in their own right in the destruction of epithelial cells, whereas B lymphocytes that predominate in severe lesions have never been implicated in direct tissue damage. Using co-culture experiments with human salivary gland (HSG) cell line cells and tonsilar B lymphocytes, we observed that direct HSG cell-B lymphocyte contacts were able to induce apoptosis in epithelial cells. This B lymphocyte-mediated cell death could not be ascribed to Fas-Fas ligand interactions but required translocation of protein kinase C delta (PKC δ) into the nucleus of epithelial cells. Ultimately, activation of PKCδ resulted in histone H2B phosphorylation on serine 14 and poly (ADP-ribose) polymerase cleavage. Our results suggest that B lymphocytes infiltrating the SGs of patients with SS could evoke epithelial cell apoptosis.
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https://hal.univ-brest.fr/hal-00771202
Contributor : Geneviève Michel <>
Submitted on : Tuesday, January 8, 2013 - 11:01:40 AM
Last modification on : Wednesday, November 29, 2017 - 2:53:52 PM

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Marie-Michèle Varin, Thomas Guerrier, Valérie Devauchelle-Pensec, Christophe Jamin, Pierre Youinou, et al.. In Sjögren's syndrome, B lymphocytes induce epithelial cells of salivary glands into apoptosis through protein kinase C delta activation.. Autoimmunity Reviews, Elsevier, 2012, 11 (4), pp.252-8. ⟨10.1016/j.autrev.2011.10.005⟩. ⟨hal-00771202⟩

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