In vitro effects of triclosan and methyl-triclosan on the marine gastropod Haliotis tuberculata. - Université de Bretagne Occidentale Accéder directement au contenu
Article Dans Une Revue Comparative Biochemistry and Physiology - Part C: Toxicology and Pharmacology Année : 2012

In vitro effects of triclosan and methyl-triclosan on the marine gastropod Haliotis tuberculata.

Résumé

Triclosan (2,4,4'-trichloro-2'-hydroxy-diphenyl ether; TCS) is an antibacterial agent incorporated in a wide variety of household and personal care products. Because of its partial elimination in sewage treatment plants, TCS is commonly detected in natural waters and sediments. Moreover, due to its high hydrophobicity, TCS accumulates in fatty tissues in various aquatic organisms. TCS can be converted into methyl-triclosan (2,4,4'-trichloro-2'-methoxydiphenyl ether; MTCS) after biological methylation. In this study, the acute cytotoxicity of TCS and MTCS in short-term in vitro experiments was assessed on cell cultures from the European abalone Haliotis tuberculata. The results showed that morphology and density of hemocyte are affected from a concentration of 8 μM TCS. Using the XTT reduction assay, TCS has been demonstrated to decrease hemocyte metabolism activity in a dose- and time-dependent exposure. The IC(50) was evaluated at 6 μM for both hemocyte and gill cells after a 24 h-incubation with TCS. A significant cytotoxicity of MTCS was also observed from 4 μM in 24 h-old hemocyte culture. Our results reveal a toxic effect of TCS and MTCS on immune (hemocytes) and/or respiratory cells (gill cells) of the abalone, species living in coastal waters areas and exposed to anthropogenic pollution.

Dates et versions

hal-00766591 , version 1 (18-12-2012)

Identifiants

Citer

Beatrice Gaume, Nathalie Bourgougnon, Stéphanie Auzoux-Bordenave, Benoit Roig, Barbara Le Bot, et al.. In vitro effects of triclosan and methyl-triclosan on the marine gastropod Haliotis tuberculata.. Comparative Biochemistry and Physiology - Part C: Toxicology and Pharmacology, 2012, 156 (2), pp.87-94. ⟨10.1016/j.cbpc.2012.04.006⟩. ⟨hal-00766591⟩
252 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More