Perkinsus marinus, a protozoan parasite of the eastern oyster, has a requirement for dietary sterols

Abstract : Perkinsus marinus, a protozoan parasite of the eastern oyster, Crassostrea virginica, causes high mortality in its host along the Atlantic and Gulf coasts of North America. P. marinus meronts cultured in vitro in medium containing complete lipid supplement (cod liver oil, cholesterol and alpha tocopherol acetate in detergent) are able to synthesize a wide variety of lipids, yet cultures cannot be maintained in lipid-free medium. To determine P marinus lipid requirements meronts were inoculated into media containing different combinations of lipid components in detergent. Treatments included complete lipid supplement (positive control), detergent only (negative control), cholesterol in detergent, a tocopherol acetate in detergent and cholesterol+alpha tocopherol acetate in detergent. Meronts proliferated in the positive control medium and media containing cholesterol or cholesterol+alpha tocopherol acetate, but failed to proliferate in the negative control medium and the medium containing just a tocopherol acetate. Gas chromatography analysis of P. marinus meronts grown in medium with added C-13 sodium acetate (0.5 mg mL(-1)) revealed the presence of fatty acids containing C-13, but the only sterol present was cholesterol containing no 1 3 C. These results suggest that R marinus cannot synthesize sterols and must sequester them from its host.
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Comparative Biochemistry and Physiology - Part A: Molecular and Integrative Physiology, Elsevier, 2007, 146 (1), pp.141-147. 〈10.1016/j.cbpa.2006.10.004〉
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http://hal.univ-brest.fr/hal-00670383
Contributeur : Catherine Bertignac <>
Soumis le : mercredi 15 février 2012 - 12:02:34
Dernière modification le : jeudi 11 janvier 2018 - 06:22:24

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Eric Lund, Fu-Lin Chu, Philippe Soudant, Ellen Harvey. Perkinsus marinus, a protozoan parasite of the eastern oyster, has a requirement for dietary sterols. Comparative Biochemistry and Physiology - Part A: Molecular and Integrative Physiology, Elsevier, 2007, 146 (1), pp.141-147. 〈10.1016/j.cbpa.2006.10.004〉. 〈hal-00670383〉

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