Vibrio aestuarianus zinc metalloprotease causes lethality in the Pacific oyster Crassostrea gigas and impairs the host cellular immune defenses

Yannick Labreuche 1, 2, * Frédérique Le Roux 3, 4 Joël Henry 5 Céline Zatylny 5 Arnaud Huvet 1 Christophe Lambert 6 Philippe Soudant 6 Didier Mazel 4 Jean-Louis Nicolas 1
* Auteur correspondant
1 PE2M - Physiologie et Ecophysiologie des Mollusques Marins
2 LEAD - Lagons, Ecosystèmes et Aquaculture Durable [Nouméa]
3 LGP - Laboratoire de Génétique et Pathologie
4 PGB - Plasticité du Génome Bactérien
5 PE2M - Physiologie et Ecophysiologie des Mollusques Marins
6 LEMAR - Laboratoire des Sciences de l'Environnement Marin (LEMAR)
Abstract : Extracellular products (ECPs) of the pathogenic Vibrio aestuarianus 01/32 were previously reported to display lethality in Crassostrea gigas oysters and to cause morphological changes and immunosuppression in oyster hemocytes. To identify the source of this toxicity, biochemical and genetic approaches were developed. ECP protease activity and lethality were shown to be significantly reduced following incubation with metal chelators, suggesting the involvement of a zinc metalloprotease. An open reading frame of 1836 bp encoding a 611-aa metalloprotease (designated yam) was identified. The deduced protein sequence showed high homology to other Vibrio metalloproteases reported to be involved in pathogenicity. To further confirm the role of this enzyme in ECP toxicity, a plasmid carrying the yarn gene under the control of an araC-P(BAD) expression cassette was transferred to a Vibrio splendidus related strain, LMC20012(T), previously characterized as non-pathogenic to oysters. Expression of Vam conferred a toxic phenotype to LMG20012(T) ECPs in vivo and cytotoxicity to oyster hemocytes in vitro. Collectively, these data suggest that the Vam metalloprotease is a major contributor to the toxicity induced by V aestuarianus ECPs and is involved in the impairment of oyster hemocyte functions.
Keywords : Extracellular products Hemocytes Metalloprotease Crassostrea gigas Oyster Vibrio aestuarianus
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Article dans une revue
Fish and Shellfish Immunology, Elsevier, 2010, 29 (5), pp.753-758. 〈10.1016/j.fsi.2010.07.007〉
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Contributeur : Catherine Bertignac <>
Soumis le : mercredi 15 février 2012 - 11:51:22
Dernière modification le : mercredi 6 juin 2018 - 01:24:53

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Yannick Labreuche, Frédérique Le Roux, Joël Henry, Céline Zatylny, Arnaud Huvet, et al.. Vibrio aestuarianus zinc metalloprotease causes lethality in the Pacific oyster Crassostrea gigas and impairs the host cellular immune defenses. Fish and Shellfish Immunology, Elsevier, 2010, 29 (5), pp.753-758. 〈10.1016/j.fsi.2010.07.007〉. 〈hal-00670371〉

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