Membrane phospholipid composition of hemocytes in the Pacific oyster Crassostrea gigas and the Manila clam Ruditapes philippinarum

Fabienne Le Grand 1 Edouard Kraffe 1, * Yanic Marty 1 Ludovic Donaghy 2 Philippe Soudant 2
* Auteur correspondant
1 CEMCA - Chimie, Electrochimie Moléculaires et Chimie Analytique
2 LEMAR - Laboratoire des Sciences de l'Environnement Marin (LEMAR)
Abstract : The detailed sterol (free sterol proportions and compositions) and phospholipid (PL) compositions (relative proportions of PL classes and subclasses and their respective fatty acid (FA) compositions) of hemocyte membranes were investigated in two bivalve mollusks: the Pacific oyster Crassostrea gigas and the Manila clam Ruditapes philippinarum. Hemocyte membrane lipids of both species revealed similar general composition: i) their free sterol/PL ratio was above 0.4 and ii) their PL were predominated by the diacyl + alkyl forms of glycerophosphatidylcholine (PC), the plasmalogen form of glycerophosphatidylethanolamine (PE) and ceramide aminoethylphosphonate (CAEP). Free sterols were predominated by cholesterol in both species. Plasmalogen forms of PE and glycerophosphatidylserine (PS) represented 82-83% and 46-55% of total PE and PS, respectively. When compared to their respective diacyl + alkyl forms, plasmalogen forms of PE and PS were specifically enriched in non-methylene-interrupted (NMI) FA and 20:1n-11, suggesting a functional significance of these PL molecular species in bivalve hemocytes. Lysoglycerophosphatidylcholine (LysoPC) levels were found to be fairly high in hemocytes, accounting for about 8% of the PL Some species-specific features were also found. LysoPC and glycerophosphatidylinositol (PI) FA compositions differed between Ruditapes philippinarum and Crassostrea gigas. CAEP proportion was higher in R. philippinarum than in C. gigas (14.5% and 27.9% of the PL respectively). Hemolymph cell monolayer observations and flow-cytometric analyses revealed species-specific hemocyte morphology and sub-populations which could account for some of the observed species-specific membrane lipid compositions.
Keywords : Plasmalogens Phospholipid classes Hemocytes Non-methylene-interrupted fatty acids Ceramide aminoethylphosphonate Crassostrea gigas Free sterols R philippinarum
Type de document :
Article dans une revue
Comparative Biochemistry and Physiology - Part A: Molecular and Integrative Physiology, Elsevier, 2011, 159 (4), pp.383-391. 〈10.1016/j.cbpa.2011.04.006〉
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http://hal.univ-brest.fr/hal-00670270
Contributeur : Catherine Bertignac <>
Soumis le : mercredi 15 février 2012 - 09:45:02
Dernière modification le : jeudi 11 janvier 2018 - 06:22:24

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Fabienne Le Grand, Edouard Kraffe, Yanic Marty, Ludovic Donaghy, Philippe Soudant. Membrane phospholipid composition of hemocytes in the Pacific oyster Crassostrea gigas and the Manila clam Ruditapes philippinarum. Comparative Biochemistry and Physiology - Part A: Molecular and Integrative Physiology, Elsevier, 2011, 159 (4), pp.383-391. 〈10.1016/j.cbpa.2011.04.006〉. 〈hal-00670270〉

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