A cDNA microarray for Crassostrea virginica and C. gigas.

Matthew J Jenny; Robert W Chapman; Annalaura Mancia; Yian A Chen; David Mckillen; Hal Trent; Paul Lang; Jean-Michel Escoubas; Evelyne Bachere; Viviane Boulo; Z John Liu; Paul S Gross; Charles Cunningham; Pauline M Cupit; Arnaud Tanguy; Ximing Guo; Dario Moraga; Isabelle Boutet; Arnaud Huvet; Sylvain de Guise; Jonas S Almeida; Gregory W Warr

doi:10.1007/s10126-007-9041-1

Journal articles

A cDNA microarray for Crassostrea virginica and C. gigas.

Matthew J Jenny ^{1, 2} Robert W Chapman ^{1, 3} Annalaura Mancia ^{1, 4} Yian A Chen ⁵ David Mckillen ^{1, 4} Hal Trent ^{1, 4} Paul Lang ⁶ Jean-Michel Escoubas ⁷ Evelyne Bachere ⁸ Viviane Boulo ⁸ Z John Liu ⁹ Paul S Gross ^{1, 4} Charles Cunningham ^{1, 10} Pauline M Cupit ^{1, 10} Arnaud Tanguy ¹¹ Ximing Guo ¹² Dario Moraga ¹³ Isabelle Boutet ¹¹ Arnaud Huvet ¹⁴ Sylvain de Guise ¹⁵ Jonas S Almeida ¹⁶ Gregory W Warr ^{1, 17}

1 Hollings Marine Laboratory

2 WHOI - Biology Department

3 South Carolina Department of Natural Resources

4 Biochemistry and Molecular Biology

5 Department of Statistics

6 Department of Fisheries and Wildlife

7 EMIP - Ecologie microbienne des insectes et interactions hôte-pathogène

8 GPIA - Génome, populations, interactions, adaptation

9 Department of Fisheries and Allied Aquacultures and Program of Cell and Molecular Biosciences

10 Department of Biology [New Mexico]

11 AD2M - Adaptation et diversité en milieu marin

12 HSRL - Haskin Shellfish Research Laboratory

13 LEMAR - Laboratoire des Sciences de l'Environnement Marin (LEMAR)

14 PE2M - Physiologie et Ecophysiologie des Mollusques Marins

15 Department of Pathobiology

16 Program in Biomathematics and Biostatistics

17 Department of Biochemistry and Molecular Biology

Abstract : The eastern oyster, Crassostrea virginica, and the Pacific oyster, C. gigas, are species of global economic significance as well as important components of estuarine ecosystems and models for genetic and environmental studies. To enhance the molecular tools available for oyster research, an international group of collaborators has constructed a 27,496-feature cDNA microarray containing 4460 sequences derived from C. virginica, 2320 from C. gigas, and 16 non-oyster DNAs serving as positive and negative controls. The performance of the array was assessed by gene expression profiling using gill and digestive gland RNA derived from both C. gigas and C. virginica, and digestive gland RNA from C. ariakensis. The utility of the microarray for detection of homologous genes by cross-hybridization between species was also assessed and the correlation between hybridization intensity and sequence homology for selected genes determined. The oyster cDNA microarray is publicly available to the research community on a cost-recovery basis.

Keywords : Crassostrea gigas bivalve Crassostrea virginica heterologous hybridization microarray oyster

Document type :

Journal articles

Domain :

Environmental Sciences

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https://hal.univ-brest.fr/hal-00669982
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Submitted on : Tuesday, February 14, 2012 - 11:48:33 AM
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A cDNA microarray for Crassostrea virginica and C. gigas.

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A cDNA microarray for Crassostrea virginica and C. gigas.

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