Identification of genes expressed in juvenile Haliotis rufescens in response to different copper concentrations in the north of Chile under controlled conditions

Fernando Silva-Aciares 1, * Manuel Zapata 2 Jennifer Tournois 3 Dario Moraga 3 Carlos Riquelme 1, 2
* Auteur correspondant
1 Laboratorio de Ecologia Microbiana
2 CICITEM - Centro Investigación Científica y Tecnológica para la Minería
3 LEMAR - Laboratoire des Sciences de l'Environnement Marin (LEMAR)
Abstract : This study reports molecular markers potentially associated with resistance or sensitivity to the impact of copper in juvenile red abalone, Haliotis rufescens, in the north of Chile under experimental conditions. Genomic analysis was made applying subtractive hybridization libraries (SSH) to identify genes up-and down regulated during cooper exposure in abalone over periods of 12 and 168 h exposed to 2.5 and 10 μg/L of Cu+2. Results obtained from the SSH library revealed 368 different sequences regulated by copper, that correspond to eight major physiological functions. The validation of these sequences obtained by SSH as well as their expression kinetics were made by PCR in real time on 14 potential genes regulated by metal stress. This study provides information for the characterization of potential genomic markers that may be used in future environmental monitoring and to investigate new mechanisms of stress to copper in this commercially important marine species.
Keywords : Haliotis rufescens Copper stress Suppression subtractive hybridization (SSH) Gene expression Real time PCR
Type de document :
Article dans une revue
Marine Pollution Bulletin, Elsevier, 2011, 62 (12), pp.2671-2680. 〈10.1016/j.marpolbul.2011.09.023〉
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http://hal.univ-brest.fr/hal-00668106
Contributeur : Catherine Bertignac <>
Soumis le : jeudi 9 février 2012 - 09:55:43
Dernière modification le : jeudi 11 janvier 2018 - 06:22:24

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Fernando Silva-Aciares, Manuel Zapata, Jennifer Tournois, Dario Moraga, Carlos Riquelme. Identification of genes expressed in juvenile Haliotis rufescens in response to different copper concentrations in the north of Chile under controlled conditions. Marine Pollution Bulletin, Elsevier, 2011, 62 (12), pp.2671-2680. 〈10.1016/j.marpolbul.2011.09.023〉. 〈hal-00668106〉

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