Skip to Main content Skip to Navigation
Journal articles

Identification of genes expressed in juvenile Haliotis rufescens in response to different copper concentrations in the north of Chile under controlled conditions

Abstract : This study reports molecular markers potentially associated with resistance or sensitivity to the impact of copper in juvenile red abalone, Haliotis rufescens, in the north of Chile under experimental conditions. Genomic analysis was made applying subtractive hybridization libraries (SSH) to identify genes up-and down regulated during cooper exposure in abalone over periods of 12 and 168 h exposed to 2.5 and 10 μg/L of Cu+2. Results obtained from the SSH library revealed 368 different sequences regulated by copper, that correspond to eight major physiological functions. The validation of these sequences obtained by SSH as well as their expression kinetics were made by PCR in real time on 14 potential genes regulated by metal stress. This study provides information for the characterization of potential genomic markers that may be used in future environmental monitoring and to investigate new mechanisms of stress to copper in this commercially important marine species.
Document type :
Journal articles
Complete list of metadatas

https://hal.univ-brest.fr/hal-00668106
Contributor : Catherine Bertignac <>
Submitted on : Thursday, February 9, 2012 - 9:55:43 AM
Last modification on : Tuesday, June 23, 2020 - 3:40:40 AM

Identifiers

Collections

Citation

Fernando Silva-Aciares, Manuel Zapata, Jennifer Tournois, Dario Moraga, Carlos Riquelme. Identification of genes expressed in juvenile Haliotis rufescens in response to different copper concentrations in the north of Chile under controlled conditions. Marine Pollution Bulletin, Elsevier, 2011, 62 (12), pp.2671-2680. ⟨10.1016/j.marpolbul.2011.09.023⟩. ⟨hal-00668106⟩

Share

Metrics

Record views

377