Optimization of free radical scavenging activity by response surface methodology in the hydrolysis of shrimp processing discards

Abstract : Response surface methodology (RSM) was used to optimize the hydrolysis conditions (temperature, pH and Alcalase® 2,4L concentration), in order to obtain the hydrolysate with the strongest antioxidant activity using the 1,1-diphenyl-2-picryl-hydrazyl (DPPHradical dot) decolouration assay. The optimum conditions obtained from experiments were pH 9.7, 66.2 °C, enzyme concentration = 68.1 Anson units (AU)/kg crude protein. The analysis of variance in RSM showed that pH and temperature (T) were the most important factors of the process (P < 0.001). The experimental conditions produced both an enzymatic and chemical protein solubilization.
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Submitted on : Wednesday, December 21, 2011 - 4:41:09 PM
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Fabienne Guérard, Maria Teresa Sumaya-Martinez, Delphine Laroque, Aurelie Chabeaud, Laurent Dufossé. Optimization of free radical scavenging activity by response surface methodology in the hydrolysis of shrimp processing discards. Process Biochemistry, Elsevier, 2007, 42 (11), pp.1486-1491. ⟨10.1016/j.procbio.2007.07.016⟩. ⟨hal-00654351⟩

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