Flow cytometric measurement of the clearance rate in the blue mussel Mytilus edulis and the development of a new individual exposure system for aquatic immunotoxicological studies

Matthieu Duchemin; Nathalie Wessel; Michel Fournier; Michel Auffret

doi:10.1016/j.envpol.2007.08.017

Flow cytometric measurement of the clearance rate in the blue mussel Mytilus edulis and the development of a new individual exposure system for aquatic immunotoxicological studies

Matthieu Duchemin ^{1, 2} Nathalie Wessel ¹ Michel Fournier ^{2, 3} Michel Auffret ¹

1 LEMAR - Laboratoire des Sciences de l'Environnement Marin (LEMAR)

2 INRS-IAF - Institut Armand Frappier

3 ISMER - Institut des Sciences de la MER de Rimouski

Abstract : Animals in poor health condition are not relevant biological models. The current study focused on the use of the clearance rate of Mytilus edulis to assess the gross physiological condition of individuals maintained in stressful experimental conditions. This approach was developed in a new, highly controlled experimental exposure device designed to investigate individual responses in aquatic ecotoxicological studies. Both clearance rate values and immune parameters analysis indicated that the health condition of mussels kept in 50 ml tubes for 24 h or 48 h was not altered compared to controls, while most parameters were depressed after 72 h. Moreover, this study confirms the relevance of flow cytometric for the measurement of clearance rate compared to techniques utilizing microscopy. Current results prompted us to perform further 24 h chemical exposure using this "in tubo" device.

Mots-clés : Aquatic pollution Individual exposure Clearance rate Mytilus edulis Immune parameters

Type de document :

Article dans une revue

Environmental Pollution, Elsevier, 2008, 153 (2), pp.492-496. 〈10.1016/j.envpol.2007.08.017〉

Domaine :

Sciences de l'environnement

Liste complète des métadonnées

http://hal.univ-brest.fr/hal-00654328
Contributeur : Catherine Bertignac <>
Soumis le : mercredi 21 décembre 2011 - 16:08:46
Dernière modification le : lundi 5 février 2018 - 15:22:10

Flow cytometric measurement of the clearance rate in the blue mussel Mytilus edulis and the development of a new individual exposure system for aquatic immunotoxicological studies

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Flow cytometric measurement of the clearance rate in the blue mussel Mytilus edulis and the development of a new individual exposure system for aquatic immunotoxicological studies

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