Characterization of reproduction-specific genes in a marine bivalve mollusc: influence of maturation stage and sex on mRNA expression.

Abstract : Numerous studies have investigated the reproduction mechanisms in mollusc species at a biochemical and physiological level; few have described these mechanisms at a molecular level, despite great commercial interest in several mollusc species. We investigated genes involved in gonad maturation of the marine scallop Argopecten purpuratus. A cDNA library was made from gonad tissue. After sequence analysis, 418 unique genes were characterized, of these, about 80% were of unknown function. Among the identified sequences, we analyzed the mRNA expression by real-time PCR of 7 genes involved in reproduction mechanisms, either directly: testis-specific serine/threonine-protein kinase (TSSK), vitellogenin (Vg), and spermatogenesis and centriole associated 1 (SCA) or indirectly: calcineurin A (CNA), centrin, RNA-specific adenosine deaminase (ADAR), and cytidine deaminase (CDA). The real-time PCR analyses were conducted on different tissues of mature and immature scallops (testis, ovary, immature gonad, gill, digestive gland and mantle). The genes studied, presented (1) a strong tissue-dependent expression pattern (higher expression in gonad tissues than in all other tissues) and (2) a sex- and maturation-specific expression pattern (except centrin). This is the first time that the expression of specific genes involved in reproduction mechanisms in a marine mollusc has been described at the molecular level.
Type de document :
Article dans une revue
Gene, Elsevier, 2008, 407 (1-2), pp.130-8. 〈10.1016/j.gene.2007.10.005〉
Liste complète des métadonnées

http://hal.univ-brest.fr/hal-00653284
Contributeur : Catherine Bertignac <>
Soumis le : lundi 19 décembre 2011 - 11:36:49
Dernière modification le : jeudi 11 janvier 2018 - 06:22:24

Identifiants

Collections

Citation

Isabelle Boutet, Dario Moraga, Luis Marinovic, Johana Obreque, Pamela Chavez-Crooker. Characterization of reproduction-specific genes in a marine bivalve mollusc: influence of maturation stage and sex on mRNA expression.. Gene, Elsevier, 2008, 407 (1-2), pp.130-8. 〈10.1016/j.gene.2007.10.005〉. 〈hal-00653284〉

Partager

Métriques

Consultations de la notice

168